A fluorescent probe for detecting H 2 O 2 and delivering H 2 S in lysosomes and its application in maintaining the redox environments.

Hydrogen peroxide (H ₂ O ₂ ) is a reactive oxygen species (ROS) that can be used as a marker for the occurrence of oxidative stress in the organism. Lysosomes serve as intracellular digestive sites, and when the concentration of H ₂ O ₂ in them is abnormal, lysosomal function is often impaired, leading to the development of diseases. Hydrogen sulfide (H ₂ S) acts as a gaseous signaling molecule that scavenges H ₂ O ₂ from cells and tissues, thereby maintaining the redox environment of the body. However, most of the reported hydrogen peroxide fluorescent probes so far can only detect H ₂ O ₂ , but cannot maintain the intracellular redox environment. In this paper, an H ₂ O ₂ fluorescent probe LN-HOD with lysosomal targeting properties was designed and synthesized by combining the H ₂ O ₂ recognition site with a naphthylamine fluorophore via a thiocarbamate moiety. The probe has the advantages of large Stokes shift (110 nm), high sensitivity and good H ₂ S release capability. The probe LN-HOD can be used to detect H ₂ O ₂ in cells, zebrafish and plant roots. In addition, LN-HOD detects changes in the concentration of H ₂ O ₂ in plant roots when Arabidopsis is stressed by cadmium ion (Cd ²⁺ ). And through its ability to release H ₂ S, it can help to remove excess H ₂ O ₂ and maintain the redox environment in cells, zebrafish and plant roots. The present work provides new ideas for the detection and assisted removal of H ₂ O ₂ , which contributes to the in-depth study of the cellular microenvironment in organisms.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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