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A fluorescent probe for detecting H 2 O 2 and delivering H 2 S in lysosomes and its application in maintaining the redox environments.
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Talanta [Talanta] 2024 Jun 01; Vol. 273, pp. 125894. Date of Electronic Publication: 2024 Mar 08. - Publication Year :
- 2024
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Abstract
- Hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ) is a reactive oxygen species (ROS) that can be used as a marker for the occurrence of oxidative stress in the organism. Lysosomes serve as intracellular digestive sites, and when the concentration of H <subscript>2</subscript> O <subscript>2</subscript> in them is abnormal, lysosomal function is often impaired, leading to the development of diseases. Hydrogen sulfide (H <subscript>2</subscript> S) acts as a gaseous signaling molecule that scavenges H <subscript>2</subscript> O <subscript>2</subscript> from cells and tissues, thereby maintaining the redox environment of the body. However, most of the reported hydrogen peroxide fluorescent probes so far can only detect H <subscript>2</subscript> O <subscript>2</subscript> , but cannot maintain the intracellular redox environment. In this paper, an H <subscript>2</subscript> O <subscript>2</subscript> fluorescent probe LN-HOD with lysosomal targeting properties was designed and synthesized by combining the H <subscript>2</subscript> O <subscript>2</subscript> recognition site with a naphthylamine fluorophore via a thiocarbamate moiety. The probe has the advantages of large Stokes shift (110 nm), high sensitivity and good H <subscript>2</subscript> S release capability. The probe LN-HOD can be used to detect H <subscript>2</subscript> O <subscript>2</subscript> in cells, zebrafish and plant roots. In addition, LN-HOD detects changes in the concentration of H <subscript>2</subscript> O <subscript>2</subscript> in plant roots when Arabidopsis is stressed by cadmium ion (Cd <superscript>2+</superscript> ). And through its ability to release H <subscript>2</subscript> S, it can help to remove excess H <subscript>2</subscript> O <subscript>2</subscript> and maintain the redox environment in cells, zebrafish and plant roots. The present work provides new ideas for the detection and assisted removal of H <subscript>2</subscript> O <subscript>2</subscript> , which contributes to the in-depth study of the cellular microenvironment in organisms.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 273
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 38461644
- Full Text :
- https://doi.org/10.1016/j.talanta.2024.125894