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[BRAF V600E expression in ameloblastomas, ameloblastic carcinomas and cysts].

Authors :
Pei J
Zhang L
Jia YX
Chen WH
Source :
Shanghai kou qiang yi xue = Shanghai journal of stomatology [Shanghai Kou Qiang Yi Xue] 2023 Dec; Vol. 32 (6), pp. 630-634.
Publication Year :
2023

Abstract

Purpose: To investigate protein and genetic status of BRAF V600E in ameloblastomas, ameloblastic carcinomas and cysts, and to explore if recurrence and malignant transformation of ameloblastomas depends on BRAF status.<br />Methods: Twenty cysts, 25 primary ameloblastomas, 25 recurrent ameloblastomas and 8 ameloblastic carcinomas were analysed by immunohistochemistry, and 2 cysts, 5 primary ameloblatomas, 5 recurrent ameloblastomas, 3 atypical ameloblastomas and 6 ameloblastic carcinomas were analysed by quantitative real-time PCR(qPCR). SPSS 26.0 software package was used for data analysis.<br />Results: There was no correlation between BRAF V600E expression and recurrence. Cysts and carcinomas had lower frequencies of BRAF V600E mutations than ameloblastomas. There was a concordance between positive staining of BRAF V600E protein and BRAF V600E mutation.<br />Conclusions: BRAF V600E mutation may be useful in the differential diagnosis of ameloblastomas with cysts and ameloblastic carcinomas. Immunohistochemistry may be a beneficial method to screen for BRAF V600E mutation in ameloblastoma.

Details

Language :
Chinese
ISSN :
1006-7248
Volume :
32
Issue :
6
Database :
MEDLINE
Journal :
Shanghai kou qiang yi xue = Shanghai journal of stomatology
Publication Type :
Academic Journal
Accession number :
38494972