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Multitarget Mechanisms of Monocarbonyl Curcuminoid Analogues against HL-60 Cancer Cells: In Vitro and Network Pharmacology-Based Approach.
- Source :
-
ACS omega [ACS Omega] 2024 Feb 27; Vol. 9 (10), pp. 11836-11847. Date of Electronic Publication: 2024 Feb 27 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- This study addressed the cytotoxic potential of four compounds: monocarbonyl curcuminoid, ethyl (2E)-2-benzylidene-3-oxobutanoate 1 , 1,2-dimethoxy-12-methyl-13H- [1,3] benzodioxolo[5,6- c ] phenanthridine 2 , 3,5-dibenzyloxybenzyl bromide 3 , and (E)-4-(4-chlorobenzylidene)-1-(4-nitrophenyl)hexan-3-one 4 . In vitro cytotoxic assays were carried out in HL-60 and BJ cells using the MTT assay along with analysis of apoptosis with the annexin V detection kit. Additional network pharmacology and docking analyses were carried out. In the in vitro assays , compounds 2 and 4 displayed significant antiproliferative effects in HL-60 cells, exhibiting IC <subscript>50</subscript> values of 5.02 and 9.50 μM, respectively. Compound 1 showed no activity, and compound 3 displayed toxicity in BJ cells. In addition, both compounds 2 and 4 induced apoptosis in HL-60 cells. Network pharmacology and docking analyses indicated that compounds 2 and 4 had synergistic effects targeting the CASP3 and PARP1 proteins. Notably, these proteins play pivotal roles in cancer-related pathways. Thus, by modulating these proteins, monocarbonyl curcuminoid has the potential to influence various cancer-related pathways. In summary, our novel findings provide valuable insights into the potential of these compounds to serve as novel anticancer therapeutic agents, warranting further mechanistic studies and clinical exploration.<br />Competing Interests: The authors declare no competing financial interest.<br /> (© 2024 The Authors. Published by American Chemical Society.)
Details
- Language :
- English
- ISSN :
- 2470-1343
- Volume :
- 9
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- ACS omega
- Publication Type :
- Academic Journal
- Accession number :
- 38496962
- Full Text :
- https://doi.org/10.1021/acsomega.3c09427