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Coupling of gene regulation and carrier modification manipulates bacterial biofilms as robust living catalysts.

Authors :
Yan CH
Zhan YF
Chen H
Herman RA
Xu Y
Khurshid M
Gong LC
You S
Wang J
Source :
Bioresource technology [Bioresour Technol] 2024 May; Vol. 399, pp. 130604. Date of Electronic Publication: 2024 Mar 16.
Publication Year :
2024

Abstract

The biofilm of an engineered strain is limited by slow growth and low yield, resulting in an unsatisfactory ability to resist external stress and promote catalytic efficiency. Here, biofilms used as robust living catalysts were manipulated through dual functionalized gene regulation and carrier modification strategies. The results showed that gene overexpression regulates the autoinducer-2 activity, extracellular polymeric substance content and colony behavior of Escherichia coli, and the biofilm yield of csgD overexpressed strains increased by 79.35 % compared to that of the wild type strains (p < 0.05). In addition, the hydrophilicity of polyurethane fibres modified with potassium dichromate increased significantly, and biofilm adhesion increased by 105.80 %. Finally, the isoquercitrin yield in the catalytic reaction of the biofilm reinforced by the csgD overexpression strain and the modified carrier was 247.85 % higher than that of the untreated group. Overall, this study has developed engineered strains biofilm with special functions, providing possibilities for catalytic applications.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1873-2976
Volume :
399
Database :
MEDLINE
Journal :
Bioresource technology
Publication Type :
Academic Journal
Accession number :
38499206
Full Text :
https://doi.org/10.1016/j.biortech.2024.130604