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Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Jun; Vol. 149, pp. 109522. Date of Electronic Publication: 2024 Mar 26. - Publication Year :
- 2024
-
Abstract
- Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as antiviral, antioxidant, and anti-inflammatory effects. In this work, we used an in vitro model based on Western blot, ROS fluorescence probe, and real-time quantitative PCR (qRT-PCR) to examine the antiviral qualities of BBR against SGIV. The outcomes demonstrated that varying BBR concentrations could significantly inhibit the replication of SGIV. In addition, BBR greatly inhibited the production of genes associated with pro-inflammatory cytokines in SGIV-infected or SGIV-uninfected GS cells based on qRT-PCR data. Subsequent investigations demonstrated that BBR suppressed the expression of the promoter activity of NF-κB and NF-κB-p65 protein. Additionally, BBR reduced the phosphorylation of ERK 1/2, JNK, and p38. Furthermore, BBR also inhibits SGIV-induced ROS production by upregulating the expression of antioxidant-related genes. In conclusion, BBR is a viable therapy option for SGIV infection due to its antiviral properties.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Inflammation immunology
Inflammation veterinary
Antiviral Agents pharmacology
DNA Virus Infections veterinary
DNA Virus Infections immunology
Ranavirus physiology
Cell Line
Berberine pharmacology
Oxidative Stress drug effects
Fish Diseases immunology
Fish Diseases virology
Virus Replication drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 149
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 38548190
- Full Text :
- https://doi.org/10.1016/j.fsi.2024.109522