Sesquiterpenoids and steroids from Eupatorium fortunei and their inhibitory effects on NO production.

Two heterodimers including a clovane-phenylpropanoid hybrid ( 1 ) and a clovane-menthane hybrid ( 2 ), five linear sesquiterpenoids incorporating a tetrahydrofuran ring ( 3 - 6 & 8 ), and four steroids ( 7 & 9 - 11 ), were separated from the ethanolic extract of a well-known aromatic and medicinal herb Eupatorium fortunei . Their structures were characterised by detailed analyses of spectroscopic data and comparison with known analogues, with seven ( 1 - 7 ) of them being described for the first time. The hybrids 1 and 2 represent the first examples of clovane type sesquiterpenoids hybridising with other class of natural products, and compounds 3 - 6 and 8 are first linear sesquiterpenyl constituents reported from the title species. All the isolates were evaluated for their inhibitory effect on the NO production induced by LPS in murine RAW264.7 macrophage cells, and 1 , 7 , 10 and 11 exhibited moderate activity with IC ₅₀ values in the range of 24.4-43.5 µM.