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Upregulation of coagulation factor V by glucocorticoid in the preovulatory follicles of zebrafish.
- Source :
-
The Journal of steroid biochemistry and molecular biology [J Steroid Biochem Mol Biol] 2024 Jul; Vol. 241, pp. 106521. Date of Electronic Publication: 2024 Apr 16. - Publication Year :
- 2024
-
Abstract
- Increased cortisol levels in the preovulatory follicular fluid suggests a role of glucocorticoid in human ovulation. However, the mechanisms through which cortisol regulates the ovulatory process remain poorly understood. In this study, we examined the upregulation of f5 mRNA by glucocorticoid and its receptor (Gr) in the preovulatory follicles of zebrafish. Our findings demonstrate a significant increase in 11β-hydroxysteroid dehydrogenase type 2 (hsd11b2), a cortisol response gene, in preovulatory follicles. Additionally, hydrocortisone exerts a dose- and time-dependent upregulation of f5 mRNA in these follicles. Importantly, this stimulatory effect is Gr-dependent, as it was completely abolished in gr <superscript>-/-</superscript> mutants. Furthermore, site-directed mutagenesis identified a glucocorticoid response element (GRE) in the promoter of zebrafish f5. Interestingly, successive incubation of hydrocortisone and the native ovulation-inducing steroid, progestin (17α,20β-dihydroxy-4-pregnen-3-one, DHP), further enhanced f5 expression in preovulatory follicles. Overall, our results indicate that the dramatic increase of f5 expression in preovulatory follicles is partially attributable to the regulation of glucocorticoid and Gr.<br />Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Female
Zebrafish Proteins genetics
Zebrafish Proteins metabolism
Ovulation drug effects
11-beta-Hydroxysteroid Dehydrogenase Type 2 metabolism
11-beta-Hydroxysteroid Dehydrogenase Type 2 genetics
Promoter Regions, Genetic
Zebrafish genetics
Zebrafish metabolism
Ovarian Follicle metabolism
Ovarian Follicle drug effects
Glucocorticoids pharmacology
Up-Regulation drug effects
Receptors, Glucocorticoid metabolism
Receptors, Glucocorticoid genetics
Hydrocortisone pharmacology
Hydrocortisone metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1220
- Volume :
- 241
- Database :
- MEDLINE
- Journal :
- The Journal of steroid biochemistry and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 38631601
- Full Text :
- https://doi.org/10.1016/j.jsbmb.2024.106521