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Leonurus japonicus Houtt. modulates neuronal apoptosis in intracerebral hemorrhage: Insights from network pharmacology and molecular docking.
- Source :
-
Journal of ethnopharmacology [J Ethnopharmacol] 2024 Aug 10; Vol. 330, pp. 118223. Date of Electronic Publication: 2024 Apr 18. - Publication Year :
- 2024
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Abstract
- Ethnopharmacological Relevance: Leonurus japonicus Houtt. (Labiatae), commonly known as Chinese motherwort, is a herbaceous flowering plant that is native to Asia. It is widely acknowledged in traditional medicine for its diuretic, hypoglycemic, antiepileptic properties and neuroprotection. Currently, Leonurus japonicus (Leo) is included in the Pharmacopoeia of the People's Republic of China. Traditional Chinese Medicine (TCM) recognizes Leo for its myriad pharmacological attributes, but its efficacy against ICH-induced neuronal apoptosis is unclear.<br />Aims of the Study: This study aimed to identify the potential targets and regulatory mechanisms of Leo in alleviating neuronal apoptosis after ICH.<br />Materials and Methods: The study employed network pharmacology, UPLC-Q-TOF-MS technique, molecular docking, pharmacodynamic studies, western blotting, and immunofluorescence techniques to explore its potential mechanisms.<br />Results: Leo was found to assist hematoma absorption, thus improving the neurological outlook in an ICH mouse model. Importantly, molecular docking highlighted JAK as Leo's potential therapeutic target in ICH scenarios. Further experimental evidence demonstrated that Leo adjusts JAK1 and STAT1 phosphorylation, curbing Bax while augmenting Bcl-2 expression.<br />Conclusion: Leo showcases potential in mitigating neuronal apoptosis post-ICH, predominantly via the JAK/STAT mechanism.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Mice
Male
Neuroprotective Agents pharmacology
Neuroprotective Agents chemistry
Neuroprotective Agents isolation & purification
Plant Extracts pharmacology
Plant Extracts chemistry
Janus Kinase 1 metabolism
STAT1 Transcription Factor metabolism
Disease Models, Animal
Molecular Docking Simulation
Apoptosis drug effects
Network Pharmacology
Leonurus chemistry
Neurons drug effects
Neurons metabolism
Cerebral Hemorrhage drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 330
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 38642624
- Full Text :
- https://doi.org/10.1016/j.jep.2024.118223