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Comprehensive Genetic Profiling Reveals Frequent Alterations of Driver Genes on the X Chromosome in Extranodal NK/T-cell Lymphoma.
- Source :
-
Cancer research [Cancer Res] 2024 Jul 02; Vol. 84 (13), pp. 2181-2201. - Publication Year :
- 2024
-
Abstract
- Extranodal NK/T-cell lymphoma (ENKTCL) is an Epstein-Barr virus (EBV)-related neoplasm with male dominance and a poor prognosis. A better understanding of the genetic alterations and their functional roles in ENKTCL could help improve patient stratification and treatments. In this study, we performed a comprehensive genetic analysis of 178 ENKTCL cases to delineate the landscape of mutations, copy number alterations (CNA), and structural variations, identifying 34 driver genes including six previously unappreciated ones, namely, HLA-B, HLA-C, ROBO1, CD58, POT1, and MAP2K1. Among them, CD274 (24%) was the most frequently altered, followed by TP53 (20%), CDKN2A (19%), ARID1A (15%), HLA-A (15%), BCOR (14%), and MSN (14%). Chromosome X losses were the most common arm-level CNAs in females (∼40%), and alterations of four X-linked driver genes (MSN, BCOR, DDX3X, and KDM6A) were more frequent in males and females harboring chromosome X losses. Among X-linked drivers, MSN was the most recurrently altered, and its expression was lost in approximately one-third of cases using immunohistochemical analysis. Functional studies of human cell lines showed that MSN disruption promoted cell proliferation and NF-κB activation. Moreover, MSN inactivation increased sensitivity to NF-κB inhibition in vitro and in vivo. In addition, recurrent deletions were observed at the origin of replication in the EBV genome (6%). Finally, by integrating the 34 drivers and 19 significant arm-level CNAs, nonnegative matrix factorization and consensus clustering identified two molecular groups with different genetic features and prognoses irrespective of clinical prognostic factors. Together, these findings could help improve diagnostic and therapeutic strategies in ENKTCL. Significance: Integrative genetic analyses and functional studies in extranodal NK/T-cell lymphoma identify frequent disruptions of X-linked drivers, reveal prognostic molecular subgroups, and uncover recurrent MSN alterations that confer sensitivity to NF-κB inhibition.<br /> (©2024 American Association for Cancer Research.)
- Subjects :
- Humans
Male
Female
DNA Copy Number Variations
Mutation
Middle Aged
Animals
Adult
Mice
Prognosis
Aged
Gene Expression Profiling
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Young Adult
Epstein-Barr Virus Infections genetics
Epstein-Barr Virus Infections virology
Epstein-Barr Virus Infections complications
Chromosomes, Human, X genetics
Lymphoma, Extranodal NK-T-Cell genetics
Lymphoma, Extranodal NK-T-Cell virology
Lymphoma, Extranodal NK-T-Cell pathology
Lymphoma, Extranodal NK-T-Cell metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1538-7445
- Volume :
- 84
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 38657099
- Full Text :
- https://doi.org/10.1158/0008-5472.CAN-24-0132