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Q3 lab-on-chip real-time PCR for the diagnosis of Leishmania infantum infection in dogs.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2024 Jun 12; Vol. 62 (6), pp. e0010424. Date of Electronic Publication: 2024 Apr 25. - Publication Year :
- 2024
-
Abstract
- Leishmaniasis is a vector-borne disease caused by many Leishmania spp. which infect humans and other mammalian hosts. Leishmania infantum is the main agent of canine leishmaniasis (CanL) whose diagnosis is usually confirmed by serological and molecular tests. This study aimed to evaluate the clinical and analytical sensitivities of a lab-on-chip (LOC) real-time PCR applied on the portable Q3-Plus V2 platform (Q3 qPCR) in the detection of L. infantum . The Q3 qPCR performance was assessed by comparing the quantification cycle (Cq) values with those obtained using the qPCR run on a CFX96 Real-Time System (CFX96 qPCR). A total of 173 DNA samples (extracted from bone marrow, lymph node, blood, buffy coat, conjunctival swab, and skin) as well as 93 non-extracted samples (NES) (bone marrow, lymph node, blood, and buffy coat) collected from dogs were tested with both systems. Serial dilutions of each representative DNA and NES sample were used to assess the analytical sensitivity of the Q3 qPCR system. Overlapping Cq values were obtained with the Q3 qPCR and CFX96 qPCR, both using DNA extracted from L. infantum promastigotes (limit of detection, <1 promastigote per milliliter) and from biological samples as well as with NES. However, the Q3 qPCR system showed a higher sensitivity in detecting L. infantum in NES as compared with the CFX96 qPCR. Our data indicate that the Q3 qPCR system could be a reliable tool for detecting L. infantum DNA in biological samples, bypassing the DNA extraction step, which represents an advance in the point-of-care diagnostic of CanL.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Dogs
Animals
Lab-On-A-Chip Devices
Molecular Diagnostic Techniques methods
DNA, Protozoan genetics
Leishmania infantum genetics
Leishmania infantum isolation & purification
Real-Time Polymerase Chain Reaction methods
Dog Diseases diagnosis
Dog Diseases parasitology
Leishmaniasis, Visceral diagnosis
Leishmaniasis, Visceral veterinary
Leishmaniasis, Visceral parasitology
Sensitivity and Specificity
Subjects
Details
- Language :
- English
- ISSN :
- 1098-660X
- Volume :
- 62
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 38661386
- Full Text :
- https://doi.org/10.1128/jcm.00104-24