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A Genome-Wide Analysis of the CEP Gene Family in Cotton and a Functional Study of GhCEP46-D05 in Plant Development.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2024 Apr 11; Vol. 25 (8). Date of Electronic Publication: 2024 Apr 11. - Publication Year :
- 2024
-
Abstract
- C-TERMINALLY ENCODED PEPTIDEs ( CEPs ) are a class of peptide hormones that have been shown in previous studies to play an important role in regulating the development and response to abiotic stress in model plants. However, their role in cotton is not well understood. In this study, we identified 54, 59, 34, and 35 CEP genes from Gossypium hirsutum (2n = 4x = 52, AD1), G. barbadense (AD2), G. arboreum (2n = 2X = 26, A2), and G. raimondii (2n = 2X = 26, D5), respectively. Sequence alignment and phylogenetic analyses indicate that cotton CEP proteins can be categorized into two subgroups based on the differentiation of their CEP domain. Chromosomal distribution and collinearity analyses show that most of the cotton CEP genes are situated in gene clusters, suggesting that segmental duplication may be a critical factor in CEP gene expansion. Expression pattern analyses showed that cotton CEP genes are widely expressed throughout the plant, with some genes exhibiting specific expression patterns. Ectopic expression of GhCEP46-D05 in Arabidopsis led to a significant reduction in both root length and seed size, resulting in a dwarf phenotype. Similarly, overexpression of GhCEP46-D05 in cotton resulted in reduced internode length and plant height. These findings provide a foundation for further investigation into the function of cotton CEP genes and their potential role in cotton breeding.
- Subjects :
- Genome, Plant
Chromosomes, Plant genetics
Arabidopsis genetics
Arabidopsis growth & development
Genome-Wide Association Study
Peptide Hormones genetics
Peptide Hormones metabolism
Plant Development genetics
Peptides genetics
Peptides metabolism
Chromosome Mapping
Genes, Plant
Gossypium genetics
Gossypium growth & development
Gossypium metabolism
Multigene Family
Gene Expression Regulation, Plant
Plant Proteins genetics
Plant Proteins metabolism
Phylogeny
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 25
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 38673820
- Full Text :
- https://doi.org/10.3390/ijms25084231