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A label-free colorimetric biosensor utilizing natural material for highly sensitive exosome detection.
- Source :
-
Talanta [Talanta] 2024 Aug 01; Vol. 275, pp. 126182. Date of Electronic Publication: 2024 Apr 28. - Publication Year :
- 2024
-
Abstract
- Exosomes, extracellular vesicles secreted by cells, play a crucial role in intercellular communication by transferring information from source cells to recipient cells. These vesicles carry important biomarkers, including nucleic acids and proteins, which provide valuable insights into the parent cells' status. As a result, exosomes have emerged as noninvasive indicators for the early diagnosis of cancer. Colorimetric biosensors have garnered significant attention due to their cost-effectiveness, simplicity, rapid response, and reproducibility. In this study, we employ sporopollenin microcapsules (SP), a natural biopolymer material derived from pollen, as a substrate for gold nanoparticles (AuNPs). By modifying the SP-Au complex with CD63 aptamers, we develop a label-free colorimetric biosensor for exosome detection. In the absence of exosomes, the SP-Au complex catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), resulting in a color change from colorless to blue. However, the addition of exosomes inhibits the catalytic activity of the SP-Au complex due to coverage of exosomes on AuNPs. This colorimetric biosensor exhibits high sensitivity and selectivity for exosome detection, with a detection limit of 10 particles/μL and a wide linear range of 10 - 10 <superscript>8</superscript> particles/μL. Additionally, the SP-Au biosensor demonstrates remarkable resistance to serum protein adsorption and excellent catalytic stability even in harsh environments, making it highly suitable for clinical diagnostics.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Humans
Tetraspanin 30 metabolism
Tetraspanin 30 analysis
Biopolymers chemistry
Biopolymers analysis
Limit of Detection
Benzidines chemistry
Aptamers, Nucleotide chemistry
Capsules chemistry
Carotenoids
Colorimetry methods
Exosomes chemistry
Biosensing Techniques methods
Gold chemistry
Metal Nanoparticles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 275
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 38701706
- Full Text :
- https://doi.org/10.1016/j.talanta.2024.126182