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Measuring response to therapy in AML: Difference from normal flow cytometry vs RQ-PCR.
- Source :
-
Methods in cell biology [Methods Cell Biol] 2024; Vol. 186, pp. 233-247. Date of Electronic Publication: 2024 Mar 14. - Publication Year :
- 2024
-
Abstract
- Multiple technologies have been used to monitor response to therapy in acute myeloid leukemia (AML) to improve detection of leukemia over the standard of practice, morphologic counting of blasts. The two techniques most frequently used in a routine clinical setting, flow cytometry and RQ-PCR, differ in their targets, sensitivity, and ability to detect residual disease. Both flow cytometry and RQ-PCR detect the expression of abnormal gene products, at the protein level or RNA level, respectively. Flow cytometry can be applied to a broad range of AML cases while RQ-PCR is limited to specific genetic abnormalities identified in subsets of AML. This article compares the results when both techniques were used in a reference laboratory to monitor AML over the course of treatment, comparing quantitative and qualitative results.<br /> (Copyright © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.)
Details
- Language :
- English
- ISSN :
- 0091-679X
- Volume :
- 186
- Database :
- MEDLINE
- Journal :
- Methods in cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 38705601
- Full Text :
- https://doi.org/10.1016/bs.mcb.2024.02.019