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Chitinase 3 like-1 activates the Akt pathway, inducing NF-κB-dependent release of pro-inflammatory cytokines and promoting the proliferative ability in nasopharyngeal carcinoma cells.
- Source :
-
Cytokine [Cytokine] 2024 Jul; Vol. 179, pp. 156631. Date of Electronic Publication: 2024 May 05. - Publication Year :
- 2024
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Abstract
- Background: Chitinase 3 like-1 (CHI3L1) has been reported to function as an oncogene in many types of cancer. However, the biological function of CHI3L1 in nasopharyngeal carcinoma (NPC) remains unknown.<br />Methods: Differentially expressed genes (DEGs) in NPC tissues in GSE64634 and GSE12452 were downloaded from Gene Expression Omnibus (GEO). CHI3L1, interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α) mRNA expression was examined by qRT-PCR. Cell proliferation was evaluated by CCK-8 and EdU incorporation assays. Western blot analysis was used to measure the changes of CHI3L1, nuclear factor-κappaB (NF-κB), and protein kinase B (Akt) pathways. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway analyses were performed using DAVID database.<br />Results: We identified 3 overlapping DEGs using Draw Venn diagram, among which CHI3L1 was chosen for the following analyses. CHI3L1 was upregulated in NPC tissues and cells. CHI3L1 silencing suppressed inflammatory response by inactivating the NF-κB pathway and inhibited cell proliferation in NPC cells. On the contrary, CHI3L1 overexpression induced inflammatory response by activating the NF-κB pathway and promoted cell proliferation in NPC cells. According to GO and KEGG analyses, CHI3L1 positive regulates Akt signaling and is enriched in the PI3K-Akt pathway. CHI3L1 knockdown inhibited the Akt pathway, and CHI3L1 overexpression activated the Akt pathway in NPC cells. Akt overexpression abolished the effects of CHI3L1 knockdown on inflammatory response, NF-κB pathway, and proliferation in NPC cells. On the contrary, Akt knockdown abolished the effects of CHI3L1 overexpression on inflammatory response, NF-κB pathway, and proliferation in NPC cells.<br />Conclusion: CHI3L1 knockdown inhibited NF-κB-dependent inflammatory response and promoting proliferation in NPC cells by inactivating the Akt pathway.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Humans
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Inflammation metabolism
Inflammation genetics
Chitinase-3-Like Protein 1 metabolism
Chitinase-3-Like Protein 1 genetics
Proto-Oncogene Proteins c-akt metabolism
NF-kappa B metabolism
Cell Proliferation
Nasopharyngeal Carcinoma metabolism
Nasopharyngeal Carcinoma genetics
Nasopharyngeal Carcinoma pathology
Signal Transduction
Cytokines metabolism
Nasopharyngeal Neoplasms metabolism
Nasopharyngeal Neoplasms genetics
Nasopharyngeal Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0023
- Volume :
- 179
- Database :
- MEDLINE
- Journal :
- Cytokine
- Publication Type :
- Academic Journal
- Accession number :
- 38710115
- Full Text :
- https://doi.org/10.1016/j.cyto.2024.156631