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Structural Characterization of Polygonatum Cyrtonema Polysaccharide and Its Immunomodulatory Effects on Macrophages.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2024 Apr 30; Vol. 29 (9). Date of Electronic Publication: 2024 Apr 30. - Publication Year :
- 2024
-
Abstract
- A neutral Polygonatum cyrtonema polysaccharide (NPCP) was isolated and purified from Polygonatum cyrtonema by various chromatographic techniques, including DEAE-52 and Sephadex-G100 chromatography. The structure of NPCP was characterized by HPLC, HPGPC, GC-MS, FT-IR, NMR, and SEM. Results showed that NPCP is composed of glucose (55.4%) and galactose (44.6%) with a molecular weight of 3.2 kDa, and the sugar chain of NPCP was →1)-α-D-Glc-(4→1)-β-D-Gal-(3→. In vitro bioactivity experiments demonstrated that NPCP significantly enhanced macrophages proliferation and phagocytosis while inhibiting the M1 polarization induced by LPS as well as the M2 polarization induced by IL-4 and IL-13 in macrophages. Additionally, NPCP suppressed the secretion of IL-6 and TNF-α in both M1 and M2 cells but promoted the secretion of IL-10. These results suggest that NPCP could serve as an immunomodulatory agent with potential applications in anti-inflammatory therapy.
- Subjects :
- Mice
Animals
Immunologic Factors pharmacology
Immunologic Factors chemistry
Immunologic Factors isolation & purification
RAW 264.7 Cells
Cytokines metabolism
Cell Proliferation drug effects
Immunomodulating Agents pharmacology
Immunomodulating Agents chemistry
Immunomodulating Agents isolation & purification
Molecular Weight
Macrophages drug effects
Macrophages metabolism
Macrophages immunology
Polygonatum chemistry
Polysaccharides pharmacology
Polysaccharides chemistry
Polysaccharides isolation & purification
Phagocytosis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 29
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 38731567
- Full Text :
- https://doi.org/10.3390/molecules29092076