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Characterization and expression profiling of buffalo IFN-lambda family.
- Source :
-
Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2024 Jun; Vol. 272, pp. 110770. Date of Electronic Publication: 2024 May 04. - Publication Year :
- 2024
-
Abstract
- Interferon lambda (IFN-λ) is an important type III interferon triggered mainly by viral infection. IFN-λ binds to their heterodimeric receptors and signals through JAK-STAT pathways similar to type I IFN. In this study, we deduced the buffalo IFN-λ sequences through the polymerase chain reaction, and then studied IFN-λ's expression patterns in different tissues, and post induction with poly I:C and live MRSA using RT-qPCR. The full-length sequences of buffalo IFN-λ3, IFN-λ receptors, and a transcript variant of IFN-λ4 were determined. IFN-λ1 is identified as a pseudogene. Virus response elements and a recombination hotspot factor was observed in the regulatory region of IFN-λ. The IFN-λ3 expressed highest in lungs and monocytes but IFN-λ4 did not. The expression of Interferon Lambda Receptor 1 was tissue specific, while Interleukin 10 Receptor subunit beta was ubiquitous. Following poly I:C induction, IFN-λ3 expression was primarily observed in epithelial cells as opposed to fibroblasts, displaying cell type-dependent expression. The cytosolic RNA sensors were expressed highest in endometrial epithelial cells, whereas the endosomal receptor was higher in fibroblasts. 2',5'-oligoadenylate synthetase expressed higher in fibroblasts, myxoma resistance protein 1 and IFN-stimulated gene 56 in epithelial cells, displaying cell-specific antiviral response of the interferon stimulated genes (ISGs). The endometrial epithelial cells expressed IFN-λ3 after live S. aureus infection indicating its importance in bacterial infection. The induction of IFN-λ3 was S. aureus isolate specific at the same multiplicity of infection (MOI). This study elucidates the IFN-λ sequences, diverse expression patterns revealing tissue specificity, and specificity in response to poly I:C and bacterial stimuli, emphasising its crucial role in innate immune response modulation.<br />Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Poly I-C pharmacology
Gene Expression Profiling veterinary
Phylogeny
Interferon Lambda
Amino Acid Sequence
Receptors, Interferon genetics
Receptors, Interferon immunology
Female
2',5'-Oligoadenylate Synthetase genetics
2',5'-Oligoadenylate Synthetase metabolism
Staphylococcus aureus immunology
Buffaloes immunology
Buffaloes genetics
Interferons genetics
Interferons immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2534
- Volume :
- 272
- Database :
- MEDLINE
- Journal :
- Veterinary immunology and immunopathology
- Publication Type :
- Academic Journal
- Accession number :
- 38735115
- Full Text :
- https://doi.org/10.1016/j.vetimm.2024.110770