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Hybrid response to SARS-CoV-2 and Neisseria meningitidis C after an OMV-adjuvanted immunization in mice and their offspring.
- Source :
-
Human vaccines & immunotherapeutics [Hum Vaccin Immunother] 2024 Dec 31; Vol. 20 (1), pp. 2346963. Date of Electronic Publication: 2024 May 15. - Publication Year :
- 2024
-
Abstract
- COVID-19, caused by SARS-CoV-2, and meningococcal disease, caused by Neisseria meningitidis , are relevant infectious diseases, preventable through vaccination. Outer membrane vesicles (OMVs), released from Gram-negative bacteria, such as N. meningitidis , present adjuvant characteristics and may confer protection against meningococcal disease. Here, we evaluated in mice the humoral and cellular immune response to different doses of receptor binding domain (RBD) of SARS-CoV-2 adjuvanted by N. meningitidis C:2a:P1.5 OMVs and aluminum hydroxide, as a combined preparation for these pathogens. The immunization induced IgG antibodies of high avidity for RBD and OMVs, besides IgG that recognized the Omicron BA.2 variant of SARS-CoV-2 with intermediary avidity. Cellular immunity showed IFN-γ and IL-4 secretion in response to RBD and OMV stimuli, demonstrating immunologic memory and a mixed Th1/Th2 response. Offspring presented transferred IgG of similar levels and avidity as their mothers. Humoral immunity did not point to the superiority of any RBD dose, but the group immunized with a lower antigenic dose (0.5 μg) had the better cellular response. Overall, OMVs enhanced RBD immunogenicity and conferred an immune response directed to N. meningitidis too.
- Subjects :
- Animals
Mice
Female
Adjuvants, Immunologic administration & dosage
COVID-19 Vaccines immunology
COVID-19 Vaccines administration & dosage
Immunity, Cellular
Immunity, Humoral
Mice, Inbred BALB C
Meningococcal Infections prevention & control
Meningococcal Infections immunology
Spike Glycoprotein, Coronavirus immunology
Adjuvants, Vaccine administration & dosage
Aluminum Hydroxide administration & dosage
Aluminum Hydroxide immunology
Immunization methods
Antibody Affinity
Antibodies, Bacterial blood
Antibodies, Bacterial immunology
Meningococcal Vaccines immunology
Meningococcal Vaccines administration & dosage
Immunologic Memory
Th1 Cells immunology
Immunoglobulin G blood
Neisseria meningitidis immunology
Antibodies, Viral blood
Antibodies, Viral immunology
COVID-19 prevention & control
COVID-19 immunology
SARS-CoV-2 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2164-554X
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Human vaccines & immunotherapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 38745461
- Full Text :
- https://doi.org/10.1080/21645515.2024.2346963