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Inferring B-cell derived T-cell receptor induced multi-epitope-based vaccine candidate against enterovirus 71: a reverse vaccinology approach.

Authors :
Swain SK
Panda S
Sahu BP
Mahapatra SR
Dey J
Sarangi R
Misra N
Source :
Clinical and experimental vaccine research [Clin Exp Vaccine Res] 2024 Apr; Vol. 13 (2), pp. 132-145. Date of Electronic Publication: 2024 Apr 30.
Publication Year :
2024

Abstract

Purpose: Enterovirus 71, a pathogen that causes hand-foot and mouth disease (HFMD) is currently regarded as an increasing neurotropic virus in Asia and can cause severe complications in pediatric patients with blister-like sores or rashes on the hand, feet, and mouth. Notwithstanding the significant burden of the disease, no authorized vaccine is available. Previously identified attenuated and inactivated vaccines are worthless over time owing to changes in the viral genome.<br />Materials and Methods: A novel vaccine construct using B-cell derived T-cell epitopes from the virulent polyprotein found the induction of possible immune response. In order to boost the immune system, a beta-defensin 1 preproprotein adjuvant with EAAAK linker was added at the N-terminal end of the vaccine sequence.<br />Results: The immunogenicity of the designed, refined, and verified prospective three-dimensional-structure of the multi-epitope vaccine was found to be quite high, exhibiting non-allergenic and antigenic properties. The vaccine candidates bound to toll-like receptor 3 in a molecular docking analysis, and the efficacy of the potential vaccine to generate a strong immune response was assessed through in silico immunological simulation.<br />Conclusion: Computational analysis has shown that the proposed multi-epitope vaccine is possibly safe for use in humans and can elicit an immune response.<br />Competing Interests: No potential conflict of interest relevant to this article was reported.<br /> (© Korean Vaccine Society.)

Details

Language :
English
ISSN :
2287-3651
Volume :
13
Issue :
2
Database :
MEDLINE
Journal :
Clinical and experimental vaccine research
Publication Type :
Academic Journal
Accession number :
38752008
Full Text :
https://doi.org/10.7774/cevr.2024.13.2.132