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Chlorogenic acid improves the development of porcine parthenogenetic embryos by regulating oxidative stress and ameliorating mitochondrial function.
- Source :
-
Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2024 May; Vol. 59 (5), pp. e14596. - Publication Year :
- 2024
-
Abstract
- Chlorogenic acid (CGA) is an effective phenolic antioxidant that can scavenge hydroxyl radicals and superoxide anions. Herein, the protective effects and mechanisms leading to CGA-induced porcine parthenogenetic activation (PA) in early-stage embryos were investigated. Our results showed that 50 μM CGA treatment during the in vitro culture (IVC) period significantly increased the cleavage and blastocyst formation rates and improved the blastocyst quality of porcine early-stage embryos derived from PAs. Then, genes related to zygotic genome activation (ZGA) were identified and investigated, revealing that CGA can promote ZGA in porcine PA early-stage embryos. Further analysis revealed that CGA treatment during the IVC period decreased the abundance of reactive oxygen species (ROS), increased the abundance of glutathione and enhanced the activity of catalase and superoxide dismutase in porcine PA early-stage embryos. Mitochondrial function analysis revealed that CGA increased mitochondrial membrane potential and ATP levels and upregulated the mitochondrial homeostasis-related gene NRF-1 in porcine PA early-stage embryos. In summary, our results suggest that CGA treatment during the IVC period helps porcine PA early-stage embryos by regulating oxidative stress and improving mitochondrial function.<br /> (© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
- Subjects :
- Animals
Blastocyst drug effects
Swine
Membrane Potential, Mitochondrial drug effects
Antioxidants pharmacology
Female
Glutathione metabolism
Oxidative Stress drug effects
Parthenogenesis drug effects
Mitochondria drug effects
Embryo Culture Techniques veterinary
Chlorogenic Acid pharmacology
Embryonic Development drug effects
Reactive Oxygen Species metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1439-0531
- Volume :
- 59
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Reproduction in domestic animals = Zuchthygiene
- Publication Type :
- Academic Journal
- Accession number :
- 38757656
- Full Text :
- https://doi.org/10.1111/rda.14596