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Development of a nucleoside-modified mRNA vaccine against clade 2.3.4.4b H5 highly pathogenic avian influenza virus.
- Source :
-
Nature communications [Nat Commun] 2024 May 23; Vol. 15 (1), pp. 4350. Date of Electronic Publication: 2024 May 23. - Publication Year :
- 2024
-
Abstract
- mRNA lipid nanoparticle (LNP) vaccines would be useful during an influenza virus pandemic since they can be produced rapidly and do not require the generation of egg-adapted vaccine seed stocks. Highly pathogenic avian influenza viruses from H5 clade 2.3.4.4b are circulating at unprecedently high levels in wild and domestic birds and have the potential to adapt to humans. Here, we generate an mRNA lipid nanoparticle (LNP) vaccine encoding the hemagglutinin (HA) glycoprotein from a clade 2.3.4.4b H5 isolate. The H5 mRNA-LNP vaccine elicits strong T cell and antibody responses in female mice, including neutralizing antibodies and broadly-reactive anti-HA stalk antibodies. The H5 mRNA-LNP vaccine elicits antibodies at similar levels compared to whole inactivated vaccines in female mice with and without prior H1N1 exposures. Finally, we find that the H5 mRNA-LNP vaccine is immunogenic in male ferrets and prevents morbidity and mortality of animals following 2.3.4.4b H5N1 challenge. Together, our data demonstrate that a monovalent mRNA-LNP vaccine expressing 2.3.4.4b H5 is immunogenic and protective in pre-clinical animal models.<br /> (© 2024. The Author(s).)
- Subjects :
- Animals
Female
Mice
Male
Antibodies, Neutralizing immunology
Mice, Inbred BALB C
Influenza in Birds prevention & control
Influenza in Birds immunology
Influenza in Birds virology
Humans
RNA, Messenger genetics
RNA, Messenger immunology
RNA, Messenger metabolism
Influenza A Virus, H1N1 Subtype immunology
Influenza A Virus, H1N1 Subtype genetics
Birds virology
Lipids chemistry
Liposomes
Influenza Vaccines immunology
Influenza Vaccines administration & dosage
Ferrets
Nanoparticles chemistry
Influenza A Virus, H5N1 Subtype immunology
Influenza A Virus, H5N1 Subtype genetics
Antibodies, Viral immunology
Hemagglutinin Glycoproteins, Influenza Virus immunology
Hemagglutinin Glycoproteins, Influenza Virus genetics
Orthomyxoviridae Infections prevention & control
Orthomyxoviridae Infections immunology
Orthomyxoviridae Infections virology
mRNA Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 38782954
- Full Text :
- https://doi.org/10.1038/s41467-024-48555-z