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Characterization, genome analysis, and therapeutic evaluation of a novel Salmonella phage vB_SalS_JNS02: a candidate bacteriophage for phage therapy.
- Source :
-
Poultry science [Poult Sci] 2024 Jul; Vol. 103 (7), pp. 103845. Date of Electronic Publication: 2024 May 13. - Publication Year :
- 2024
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Abstract
- Phage therapy is gaining momentum as an alternative to antibiotics in the treatment of salmonellosis caused by Salmonella. In this study, a novel Salmonella phage, vB&#95;SalS&#95;JNS02, was isolated successfully from poultry farms in Shandong, China. The biological characteristics of vB&#95;SalS&#95;JNS02 were analysed, which revealed a short latent period of approximately 10 min and a burst size of 110 PFU/cell. Moreover, vB&#95;SalS&#95;JNS02 exhibited remarkable stability across a wide pH range (pH 3-12) and temperatures ranging from 30 to 80°C. Genome sequencing analysis provided valuable insights into the genetic composition of vB&#95;SalS&#95;JNS02, which consists of a double-stranded DNA genome that spans 42,450 base pairs and has a G + C content of 49.4%. Of significant importance, the genomic sequence of vB&#95;SalS&#95;JNS02 did not contain any genes related to lysogenicity, virulence, or antibiotic resistance. The phage's efficacy was evaluated in a larval challenge study. Treatment with the phage resulted in increased survival of Galleria mellonella larvae (100, 70, and 85%) (MOI 0.1) in the prophylactic treatment, co-infection treatment, and remedial treatment experiments, respectively. Another in vivo experiment investigated the potential application of the phage in broiler chickens and revealed that a single oral dose of vB&#95;SalS&#95;JNS02 (10 <superscript>8</superscript> PFU/mL, 100 µL/chick) administered 3 h after S. enteritidis oral administration provided effective protection. The introduction of bacteriophage not only enhances the production of secretory immunoglobulin A (sIgA), but also induces alterations in the composition of the gut microbial community. Phage therapy increases the relative abundance of beneficial bacteria, which helps to maintain intestinal barrier homeostasis. However, it is unable to fully restore the disrupted intestinal microbiome caused by S. enteritidis infection. Importantly, no significant adverse effects were observed in the animal subjects following oral administration of the phage, and our findings highlight vB&#95;SalS&#95;JNS02 is a hopeful candidate as a promising tool to target Salmonella infections in poultry.<br />Competing Interests: DISCLOSURES The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Lulu Li reports financial support was provided by Ministry of Science and Technology of the People's Republic of China. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Moths virology
Moths microbiology
China
Larva microbiology
Larva virology
Phage Therapy veterinary
Salmonella Phages physiology
Salmonella Phages genetics
Poultry Diseases therapy
Poultry Diseases microbiology
Poultry Diseases virology
Chickens
Salmonella Infections, Animal therapy
Salmonella Infections, Animal microbiology
Genome, Viral
Subjects
Details
- Language :
- English
- ISSN :
- 1525-3171
- Volume :
- 103
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Poultry science
- Publication Type :
- Academic Journal
- Accession number :
- 38788486
- Full Text :
- https://doi.org/10.1016/j.psj.2024.103845