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PdPt@SnS 2 Nanosheets for a Novel Ultrasensitive Electrochemiluminescence Biosensor for miRNA-21 Assay.
- Source :
-
Analytical chemistry [Anal Chem] 2024 Jun 11; Vol. 96 (23), pp. 9653-9658. Date of Electronic Publication: 2024 May 28. - Publication Year :
- 2024
-
Abstract
- PdPt nanosheets decorated on SnS <subscript>2</subscript> nanosheets (i.e., PdPt@SnS <subscript>2</subscript> NSs) were fabricated for a novel electrochemiluminescence (ECL) biosensor for ultrasensitive detection of miRNA-21 based on catalytic hairpin assembly (CHA) cycles. The PdPt@SnS <subscript>2</subscript> NSs serve as both the main luminophore and a highly effective coreaction accelerator in the ECL biosensor. In the CHA cycles, more miRNA-21 is captured, and the performance of the ECL biosensor is improved. When miRNA-21 is present, the hairpin chain DNA1 (i.e., H1) is opened, and the ferrocene (Fc)-modified hairpin chain DNA2 (i.e., Fc-H2) hybridizes with as-opened H1 by replacing miRNA-21 to stimulate CHA cycles of miRNA-21. During the CHA cycles, Fc-H2 quenches the ECL signal to monitor miRNA-21. As a result, the ECL biosensor shows ultrasensitive and highly selective detection of miRNA-21 from 1 aM to 1 nM with a detection limit (LOD) of 0.02 aM. In addition, the ECL biosensor exhibits excellent practicality for miRNA-21 detection in human serum samples.
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 96
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 38807045
- Full Text :
- https://doi.org/10.1021/acs.analchem.4c01512