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Structural and Property Characterizations of Dual-Responsive Core-Shell Tecto Dendrimers for Tumor Penetration and Gene Delivery Applications.
- Source :
-
Macromolecular rapid communications [Macromol Rapid Commun] 2024 Sep; Vol. 45 (17), pp. e2400251. Date of Electronic Publication: 2024 Jun 10. - Publication Year :
- 2024
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Abstract
- Core-shell tecto dendrimers (CSTDs) with excellent physicochemical properties and good tumor penetration and gene transfection efficiency have been demonstrated to have the potential to replace high-generation dendrimers in biomedical applications. However, their characterization and related biological properties of CSTDs for enhanced tumor penetration and gene delivery still lack in-depth investigation. Herein, three types of dual-responsive CSTDs are designed for thorough physicochemical characterization and investigation of their tumor penetration and gene delivery efficiency. Three types of CSTDs are prepared through phenylborate ester bonds of phenylboronic acid (PBA)-decorated generation 5 (G5) poly(amidoamine) (PAMAM) dendrimers as cores and monose (galactose, glucose, or mannose)-conjugated G3 PAMAM dendrimers as shells and thoroughly characterized via NMR and other techniques. It is shown that the produced CSTDs display strong correlation signals between the PBA and monose protons, similar hydrodynamic diameters, and dual reactive oxygen species- and pH-responsivenesses. The dual-responsive CSTDs are proven to have structure-dependent tumor penetration property and gene delivery efficiency in terms of small interference RNA for gene silencing and plasmid DNA for gene editing, thus revealing a great potential for different biomedical applications.<br /> (© 2024 Wiley‐VCH GmbH.)
- Subjects :
- Humans
Neoplasms drug therapy
Neoplasms therapy
Plasmids chemistry
Plasmids genetics
Plasmids metabolism
DNA chemistry
DNA genetics
Molecular Structure
Hydrogen-Ion Concentration
Boronic Acids chemistry
Transfection methods
Reactive Oxygen Species metabolism
Reactive Oxygen Species chemistry
Dendrimers chemistry
Gene Transfer Techniques
Subjects
Details
- Language :
- English
- ISSN :
- 1521-3927
- Volume :
- 45
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Macromolecular rapid communications
- Publication Type :
- Academic Journal
- Accession number :
- 38813898
- Full Text :
- https://doi.org/10.1002/marc.202400251