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Intestinal lymphatic transport of Smilax china L. pectic polysaccharide via Peyer's patches and its uptake and transport mechanisms in mononuclear phagocytes.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2024 Sep 01; Vol. 339, pp. 122256. Date of Electronic Publication: 2024 May 12. - Publication Year :
- 2024
-
Abstract
- Recently, the intestinal lymphatic transport based on Peyer's patches (PPs) is emerging as a promising absorption pathway for natural polysaccharides. Herein, the aim of this study is to investigate the PP-based oral absorption of a pectic polysaccharide from Smilax china L. (SCLP), as well as its uptake and transport mechanisms in related immune cells. Taking advantages of the traceability of fluorescently labeled SCLP, we confirmed that SCLP could be absorbed into PPs and captured by their mononuclear phagocytes (dendritic cells and macrophages) following oral administration. Subsequently, the systematic in vitro study suggested that the endocytic mechanisms of SCLP by model mononuclear phagocytes (BMDCs and RAW264.7 cells) mainly involved caveolae-mediated endocytosis, macropinocytosis and phagocytosis. More importantly, SCLP directly binds and interacts with toll-like receptor 2 (TLR2) and galectin 3 (Gal-3) receptor, and was taken up by mononuclear phagocytes in receptor-mediated manner. After internalization, SCLP was intracellularly transported primarily through endolysosomal pathway and ultimately localized in lysosomes. In summary, this work reveals novel information and perspectives about the in vivo fate of SCLP, which will contribute to further research and utilization of SCLP and other pectic polysaccharides.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Mice
RAW 264.7 Cells
Endocytosis
Pectins chemistry
Pectins metabolism
Macrophages metabolism
Macrophages drug effects
Phagocytosis drug effects
Phagocytes metabolism
Phagocytes drug effects
Toll-Like Receptor 2 metabolism
Mice, Inbred BALB C
Male
Dendritic Cells metabolism
Dendritic Cells drug effects
Administration, Oral
Peyer's Patches metabolism
Smilax chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 339
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 38823922
- Full Text :
- https://doi.org/10.1016/j.carbpol.2024.122256