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Electrochemical biosensor for sensitive detection of SARS-CoV-2 gene fragments using Bi 2 Se 3 topological insulator.
- Source :
-
Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2024 Oct; Vol. 159, pp. 108748. Date of Electronic Publication: 2024 May 26. - Publication Year :
- 2024
-
Abstract
- In this study, we have designed an electrochemical biosensor based on topological material Bi <subscript>2</subscript> Se <subscript>3</subscript> for the sensitive detection of SARS-CoV-2 in the COVID-19 pandemic. Flake-shaped Bi <subscript>2</subscript> Se <subscript>3</subscript> was obtained directly from high-quality single crystals using mechanical exfoliation, and the single-stranded DNA was immobilized onto it. Under optimal conditions, the peak current of the differential pulse voltammetry method exhibited a linear relationship with the logarithm of the concentration of target-complementary-stranded DNA, ranging from 1.0 × 10 <superscript>-15</superscript> to 1.0 × 10 <superscript>-11</superscript> M, with a detection limit of 3.46 × 10 <superscript>-16</superscript> M. The topological material Bi <subscript>2</subscript> Se <subscript>3</subscript> , with Dirac surface states, enhanced the signal-to-interference plus noise ratio of the electrochemical measurements, thereby improving the sensitivity of the sensor. Furthermore, the electrochemical sensor demonstrated excellent specificity in recognizing RNA. It can detect complementary RNA by amplifying and transcribing the initial DNA template, with an initial DNA template concentration ranging from 1.0 × 10 <superscript>-18</superscript> to 1.0 × 10 <superscript>-15</superscript> M. Furthermore, the sensor also effectively distinguished negative and positive results by detecting splitting-synthetic SARS-CoV-2 pseudovirus with a concentration of 1 copy/μL input. Our work underscores the immense potential of the electrochemical sensing platform based on the topological material Bi <subscript>2</subscript> Se <subscript>3</subscript> in the detection of pathogens during the rapid spread of acute infectious diseases.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Humans
DNA, Single-Stranded chemistry
DNA, Single-Stranded genetics
RNA, Viral genetics
RNA, Viral analysis
Selenium Compounds chemistry
Biosensing Techniques methods
SARS-CoV-2 genetics
SARS-CoV-2 isolation & purification
COVID-19 diagnosis
COVID-19 virology
Bismuth chemistry
Electrochemical Techniques methods
Limit of Detection
Subjects
Details
- Language :
- English
- ISSN :
- 1878-562X
- Volume :
- 159
- Database :
- MEDLINE
- Journal :
- Bioelectrochemistry (Amsterdam, Netherlands)
- Publication Type :
- Academic Journal
- Accession number :
- 38824746
- Full Text :
- https://doi.org/10.1016/j.bioelechem.2024.108748