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IgG sialylation occurs in B cells pre antibody secretion.
- Source :
-
Frontiers in immunology [Front Immunol] 2024 May 17; Vol. 15, pp. 1402000. Date of Electronic Publication: 2024 May 17 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Sialic acids as terminal sugar residues on cell surface or secreted proteins have many functional roles. In particular, the presence or absence of α2,6-linked sialic acid residues at the immunoglobulin G (IgG) Fc fragment can switch IgG effector functions from pro- to anti-inflammatory activity. IgG glycosylation is considered to take place inside the plasma blast/plasma cell while the molecule travels through the endoplasmic reticulum and Golgi apparatus before being secreted. However, more recent studies have suggested that IgG sialylation may occur predominantly post-antibody secretion. To what extent this extracellular IgG sialylation process contributes to overall IgG sialylation remains unclear, however. By generating bone marrow chimeric mice with a B cell-specific deletion of ST6Gal1, the key enzyme required for IgG sialylation, we now show that sialylation of the IgG Fc fragment exclusively occurs within B cells pre-IgG secretion. We further demonstrate that B cells expressing ST6Gal1 have a developmental advantage over B cells lacking ST6Gal1 expression and thus dominate the plasma cell pool and the resulting serum IgG population in mouse models in which both ST6Gal1-sufficient and -deficient B cells are present.<br />Competing Interests: Authors MH, OZ, and GL were employed by Genos Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.<br /> (Copyright © 2024 Werner, Hanić, Zaitseva, Lauc, Lux, Nitschke and Nimmerjahn.)
- Subjects :
- Animals
Mice
Mice, Knockout
Glycosylation
Mice, Inbred C57BL
Immunoglobulin Fc Fragments immunology
Immunoglobulin Fc Fragments metabolism
Immunoglobulin Fc Fragments genetics
beta-D-Galactoside alpha 2-6-Sialyltransferase
Plasma Cells immunology
Plasma Cells metabolism
Antibody Formation
Immunoglobulin G immunology
Immunoglobulin G metabolism
Sialyltransferases metabolism
Sialyltransferases genetics
B-Lymphocytes immunology
B-Lymphocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 15
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 38827747
- Full Text :
- https://doi.org/10.3389/fimmu.2024.1402000