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Overexpressing CYP81D11 enhances 2,4,6-trinitrotoluene tolerance and removal efficiency in Arabidopsis.
- Source :
-
Physiologia plantarum [Physiol Plant] 2024 May-Jun; Vol. 176 (3), pp. e14364. - Publication Year :
- 2024
-
Abstract
- Phytoremediation is a promising technology for removing the high-toxic explosive 2,4,6-trinitrotoluene (TNT) pollutant from the environment. Mining dominant genes is the key research direction of this technology. Most previous studies have focused on the detoxification of TNT rather than plants' TNT tolerance. Here, we conducted a transcriptomic analysis of wild type Arabidopsis plants under TNT stress and found that the Arabidopsis cytochrome P450 gene CYP81D11 was significantly induced in TNT-treated plants. Under TNT stress, the root length was approximately 1.4 times longer in CYP81D11-overexpressing transgenic plants than in wild type plants. The half-removal time for TNT was much shorter in CYP81D11-overexpressing transgenic plants (1.1 days) than in wild type plants (t <subscript>1/2</subscript> = 2.2 day). In addition, metabolic analysis showed no difference in metabolites in transgenic plants compared to wild type plants. These results suggest that the high TNT uptake rates of CYP81D11-overexpressing transgenic plants were most likely due to increased tolerance and biomass rather than TNT degradation. However, CYP81D11-overexpressing plants were not more tolerant to osmotic stresses, such as salt or drought. Taken together, our results indicate that CYP81D11 is a promising target for producing bioengineered plants with high TNT removing capability.<br /> (© 2024 Scandinavian Plant Physiology Society.)
- Subjects :
- Plant Roots genetics
Plant Roots metabolism
Stress, Physiological genetics
Arabidopsis genetics
Arabidopsis metabolism
Trinitrotoluene metabolism
Plants, Genetically Modified
Cytochrome P-450 Enzyme System metabolism
Cytochrome P-450 Enzyme System genetics
Gene Expression Regulation, Plant
Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
Biodegradation, Environmental
Subjects
Details
- Language :
- English
- ISSN :
- 1399-3054
- Volume :
- 176
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Physiologia plantarum
- Publication Type :
- Academic Journal
- Accession number :
- 38837226
- Full Text :
- https://doi.org/10.1111/ppl.14364