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PRDX1 exerts a photoprotection effect by inhibiting oxidative stress and regulating MAPK signaling on retinal pigment epithelium.
- Source :
-
BMC ophthalmology [BMC Ophthalmol] 2024 Jun 06; Vol. 24 (1), pp. 237. Date of Electronic Publication: 2024 Jun 06. - Publication Year :
- 2024
-
Abstract
- Background: The purpose of this study was to investigate the photoprotection effect of peroxiredoxin 1 (PRDX1) protein in ultraviolet B (UVB) irradiation-induced damage of retinal pigment epithelium (RPE) and its possible molecular mechanism.<br />Methods: ARPE-19 cell viability and apoptosis were assessed by MTT assay and flow cytometry, respectively. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the PRDX1 expression. The corresponding kits were employed to measure the levels or activities of lactate dehydrogenase (LDH), 8-hydroxy-2-deoxyguanosine (8-OHdG), reactive oxygen species (ROS), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD). Western blotting was applied to examine PRDX1 expression and mitogen-activated protein kinase (MAPK) signaling pathway-related proteins.<br />Results: After exposure to 20 mJ/cm <superscript>2</superscript> intensity of UVB irradiation for 24 h, ARPE-19 cells viability was decreased, the leakage degree of LDH and 8-OHdG were increased, and cell apoptosis was elevated. The expression of PRDX1 was significantly down-regulated in UVB-induced ARPE-19 cells. The low expression of PRDX1 was involved in high irradiation intensity. Overexpression of PRDX1 increased cell activity, decreased cell apoptosis, and LDH as well as 8-OHdG leakage in UVB-induced ARPE-19 cells. In addition to alleviating UVB-induced cell damage, PRDX1 overexpression also inhibited UVB-induced oxidative stress (down-regulation of ROS and MDA levels, up-regulation of GSH-Px and SOD activities) and the activation of MAPK signaling pathway in ARPE-19 cells.<br />Conclusion: PRDX1 exerts a photoprotection effect on RPE by attenuating UVB-induced cell damage and inhibiting oxidative stress, which can be attributed to the inhibition of MAPK signaling pathway activation.<br /> (© 2024. The Author(s).)
- Subjects :
- Humans
MAP Kinase Signaling System physiology
Cell Line
Blotting, Western
Cells, Cultured
8-Hydroxy-2'-Deoxyguanosine metabolism
Signal Transduction
Oxidative Stress
Retinal Pigment Epithelium radiation effects
Retinal Pigment Epithelium metabolism
Peroxiredoxins metabolism
Ultraviolet Rays adverse effects
Cell Survival
Apoptosis
Reactive Oxygen Species metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2415
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC ophthalmology
- Publication Type :
- Academic Journal
- Accession number :
- 38844903
- Full Text :
- https://doi.org/10.1186/s12886-024-03489-4