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Effects of Bifidobacterium animalis subsp. lactis BB-12 and yogurt on mice during oral antibiotic administration.
- Source :
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Microbiological research [Microbiol Res] 2024 Sep; Vol. 286, pp. 127794. Date of Electronic Publication: 2024 May 31. - Publication Year :
- 2024
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Abstract
- Probiotics have the potential to prevent disruptions to normal gastrointestinal function caused by oral antibiotic use. In this study, we examined the capacity of Bifidobacterium animalis subspecies lactis BB-12 (BB-12) and yogurt, separately and combined, to mitigate the effects of the antibiotic amoxicillin-clavulanate (AMC) on the gut microbiota and metabolomes of C57BL/6 J mice. Male and female mice were administered either BB-12, yogurt, BB-12 in yogurt, or saline for 10 days concurrent with the inclusion of AMC in the drinking water. Male mice exposed to AMC exhibited significant reductions (p<0.05) in body weight over the course of the study compared to sham (no AMC) controls whereas no such effects were observed for female mice. AMC administration resulted in rapid alterations to the intestinal microbiota in both sexes irrespective of BB-12 or yogurt treatment, including significant (p<0.05) losses in bacterial cell numbers and changes in microbial alpha-diversity and beta-diversity in the feces and cecal contents. The effects of AMC on the gut microbiota were observed within one day of administration and the bacterial contents continued to change over time, showing a succession marked by rapid reductions in Muribaculaceae and Lachnospiraceae and temporal increases in proportions of Acholeplasmataceae (day 1) and Streptococcaceae and Leuconostocaceae (day 5). By day 10 of AMC intake, high proportions of Gammaproteobacteria assigned as Erwiniaceae or Enterobacteriaceae (average of 63 %), were contained in the stools and were similarly enriched in the cecum. The cecal contents of mice given AMC harbored significantly reduced concentrations of (branched) short-chain fatty acids (SCFA), aspartate, and other compounds, whereas numerous metabolites, including formate, lactate, and several amino acids and amino acid derivatives were significantly enriched. Despite the extensive impact of AMC, starting at day 7 of the study, the body weights of male mice given yogurt or BB-12 (in saline) with AMC were similar to the healthy controls. BB-12 (in saline) and yogurt intake was associated with increased Streptococcaceae and both yogurt and BB-12 resulted in lower proportions of Erwiniaceae in the fecal and cecal contents. The cecal contents of mice fed BB-12 in yogurt contained levels of formate, glycine, and glutamine that were equivalent to the sham controls. These findings highlight the potential of BB-12 and yogurt to mitigate antibiotic-induced gut dysbiosis.<br />Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that influenced the work reported in this paper.<br /> (Copyright © 2024 The Authors. Published by Elsevier GmbH.. All rights reserved.)
- Subjects :
- Animals
Female
Male
Administration, Oral
Mice
Amoxicillin-Potassium Clavulanate Combination administration & dosage
Amoxicillin-Potassium Clavulanate Combination pharmacology
Cecum microbiology
Body Weight drug effects
Bacteria classification
Bacteria drug effects
Bacteria isolation & purification
Metabolome drug effects
Yogurt microbiology
Gastrointestinal Microbiome drug effects
Anti-Bacterial Agents pharmacology
Anti-Bacterial Agents administration & dosage
Bifidobacterium animalis
Probiotics administration & dosage
Mice, Inbred C57BL
Feces microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0623
- Volume :
- 286
- Database :
- MEDLINE
- Journal :
- Microbiological research
- Publication Type :
- Academic Journal
- Accession number :
- 38852301
- Full Text :
- https://doi.org/10.1016/j.micres.2024.127794