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Characterization of the novel phage vB_BceP_LY3 and its potential role in controlling Bacillus cereus in milk and rice.
- Source :
-
International journal of food microbiology [Int J Food Microbiol] 2024 Aug 16; Vol. 421, pp. 110778. Date of Electronic Publication: 2024 Jun 03. - Publication Year :
- 2024
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Abstract
- Bacillus cereus is a foodborne pathogen that induces vomiting and diarrhea in affected individuals. It exhibits resistance to traditional sterilization methods and has a high contamination rate in dairy products and rice. Therefore, the development of a new food safety controlling strategy is necessary. In this research, we isolated and identified a novel phage named vB&#95;BceP&#95;LY3, which belongs to a new genus of the subfamily Northropvirinae. This phage demonstrates a short latency period and remains stable over a wide range of temperatures (4-60 °C) and pH levels (4-11). The 28,124 bp genome of LY3 does not contain any antibiotic-resistance genes or virulence factors. With regards to its antibacterial properties, LY3 not only effectively inhibits the growth of B. cereus in TSB (tryptic soy broth), but also demonstrates significant inhibitory effects in various food matrices. Specifically, LY3 treatment at 4 °C with a high MOI (MOI = 10,000) can maintain B. cereus levels below the detection limit for up to 24 h in milk. LY3 represents a safe and promising biocontrol agent against B. cereus, possessing long-term antibacterial capabilities and stability.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Genome, Viral
Food Contamination prevention & control
Food Contamination analysis
Bacillus Phages genetics
Bacillus Phages isolation & purification
Bacillus Phages classification
Bacillus Phages physiology
Bacteriophages genetics
Bacteriophages isolation & purification
Bacteriophages physiology
Oryza microbiology
Bacillus cereus virology
Milk microbiology
Food Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3460
- Volume :
- 421
- Database :
- MEDLINE
- Journal :
- International journal of food microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 38861847
- Full Text :
- https://doi.org/10.1016/j.ijfoodmicro.2024.110778