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First Record of the Isolation of blaNDM-1-Producing Enterotoxigenic Enterobacter cloacae ssp. cloacae in Baghdad/Iraq.
- Source :
-
Clinical laboratory [Clin Lab] 2024 Jun 01; Vol. 70 (6). - Publication Year :
- 2024
-
Abstract
- Background: Antibiotic resistance is a major problem threatening human beings. The genetic determinants that carry resistance genes can be transmitted in several ways in clinical and food environments. Hence, this research study aimed to investigate the presence of New Delhi metallo-beta-lactamase-1 (blaNDM-1) produced by enterotoxigenic Enterobacter cloacae in both clinical and food samples.<br />Methods and Results: Twenty-four isolates of Enterobacter spp. were isolated, seven isolates from food samples and 17 isolates from blood taken from neonates and children (1 day - 10 years old) resident in a children's hospital. Antibiotic susceptibility test to 14 antibiotics was performed for all isolates. Enterotoxigenicity of the clinical and foodborne isolates was detected phenotypically using Suckling mouse bioassay. Genomic deoxyribonucleic acid (DNA) was extracted from the isolated Enterobacter spp. that were detected resistant to imipenem. Polymerase chain reaction (PCR) was used to amplify blaNDM-1 gene followed by sequencing. The results of the bioassay revealed that 64.28% of E. cloacae ssp. cloacae isolates were enterotoxigenic. Two E. cloacae ssp. cloacae were imipenem resistant.<br />Conclusions: This study showed that one isolate from a male child 1 < year was bla NDM-1 positive that was con-firmed by sequencing. This is the first report that revealed blaNDM-1 producing Enterobacter cloacae in Iraq.
- Subjects :
- Humans
Infant
Child
Child, Preschool
Animals
Infant, Newborn
Iraq
Food Microbiology
Mice
beta-Lactamases genetics
Enterobacter cloacae genetics
Enterobacter cloacae isolation & purification
Enterobacter cloacae drug effects
Enterobacter cloacae enzymology
Microbial Sensitivity Tests
Enterobacteriaceae Infections microbiology
Enterobacteriaceae Infections diagnosis
Anti-Bacterial Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1433-6510
- Volume :
- 70
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Clinical laboratory
- Publication Type :
- Academic Journal
- Accession number :
- 38868883
- Full Text :
- https://doi.org/10.7754/Clin.Lab.2024.230926