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A proximity ligation hybridization triggered structure-switching based signal amplification strategy for sensitive and accurate exosome detection.

Authors :
Yang W
Yang J
Zhou N
Wang Y
Source :
Analytical methods : advancing methods and applications [Anal Methods] 2024 Jul 04; Vol. 16 (26), pp. 4262-4267. Date of Electronic Publication: 2024 Jul 04.
Publication Year :
2024

Abstract

Exosomes have significant functions in intercellular communication, as well as in tumor migration and invasion. Nevertheless, the precise identification of exosomes poses a significant obstacle due to their low abundance in biofluids and potential disruption caused by free protein molecules, such as CD63 protein. In this study, we have developed a signal amplification method for precise detection of exosomes using a proximity ligation hybridization triggered structure-switching approach. The method involves the dual-recognition of exosomes by two probes: an aptamer probe that recognizes the exosomal surface protein CD63 (L1 probe), and a cholesterol probe that targets the biolipid layer of the exosomes (L2 probe). Based on the dual-recognition of exosomes, we have successfully developed an accurate and sensitive approach that integrates the proximity ligation hybridization technique with a structure-switching based signal cycle. This approach allows for the simultaneous analysis of two biomarkers, enabling both quantification and tracing of exosomes without the need for enzymes. Eventually, the proposed method exhibits a wide detection range of 5 orders of magnitude and a low limit of detection of 36 particles per μL, making it suitable for a wide range of applications in the fields of biological science, biomedical engineering, and personalized medicine.

Details

Language :
English
ISSN :
1759-9679
Volume :
16
Issue :
26
Database :
MEDLINE
Journal :
Analytical methods : advancing methods and applications
Publication Type :
Academic Journal
Accession number :
38884118
Full Text :
https://doi.org/10.1039/d4ay00829d