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Diversity and evolution of nitric oxide reduction in bacteria and archaea.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jun 25; Vol. 121 (26), pp. e2316422121. Date of Electronic Publication: 2024 Jun 20. - Publication Year :
- 2024
-
Abstract
- Nitrous oxide is a potent greenhouse gas whose production is catalyzed by nitric oxide reductase (NOR) members of the heme-copper oxidoreductase (HCO) enzyme superfamily. We identified several previously uncharacterized HCO families, four of which (eNOR, sNOR, gNOR, and nNOR) appear to perform NO reduction. These families have novel active-site structures and several have conserved proton channels, suggesting that they might be able to couple NO reduction to energy conservation. We isolated and biochemically characterized a member of the eNOR family from the bacterium Rhodothermus marinus and found that it performs NO reduction. These recently identified NORs exhibited broad phylogenetic and environmental distributions, greatly expanding the diversity of microbes in nature capable of NO reduction. Phylogenetic analyses further demonstrated that NORs evolved multiple times independently from oxygen reductases, supporting the view that complete denitrification evolved after aerobic respiration.<br />Competing Interests: Competing interests statement:The authors declare no competing interest.
- Subjects :
- Archaea metabolism
Archaea genetics
Rhodothermus metabolism
Rhodothermus enzymology
Rhodothermus genetics
Evolution, Molecular
Bacteria metabolism
Bacteria genetics
Bacterial Proteins metabolism
Bacterial Proteins genetics
Bacterial Proteins chemistry
Nitric Oxide metabolism
Oxidoreductases metabolism
Oxidoreductases genetics
Phylogeny
Oxidation-Reduction
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 121
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 38900790
- Full Text :
- https://doi.org/10.1073/pnas.2316422121