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Nasally delivered chitosan-coated poly(lactide-co-glycolide) encapsulating honeybee venom enhances T helper 1-related immunity against Salmonella Typhimurium infection in pigs.
- Source :
-
American journal of veterinary research [Am J Vet Res] 2024 Jun 20, pp. 1-10. Date of Electronic Publication: 2024 Jun 20. - Publication Year :
- 2024
- Publisher :
- Ahead of Print
-
Abstract
- Objective: Salmonella Typhimurium is a significant zoonotic concern for human food poisoning and a substantial economic burden in the swine industry. We previously reported that nasally delivered chitosan-coated poly(lactide-co-glycolide) (PLGA) encapsulating honeybee venom (CP-HBV) could enhance CD4+ T helper 1 (Th1)-related immune responses in healthy pigs. Building upon these findings, the current study aimed to investigate the protective immune enhancement by nasally delivered CP-HBV in pigs challenged with S Typhimurium.<br />Animals: 36 healthy, 4-week-old, female, Landrace X Yorkshire X Duroc pigs.<br />Methods: 36 pigs were allocated into 3 groups: CP-HBV (n = 16), control (n = 16), and healthy baseline control (n = 4). CP-HBV and control groups were challenged with S Typhimurium 7 days post-treatment. Pigs from the healthy control group were sacrificed at 0 days postinfection (DPI), and 4 pigs from each of the control and CP-HBV groups were sacrificed at 1, 2, 4, and 7 DPI. Salmonella shedding, immune cell frequencies, cytokines, and transcriptional factor expression levels were measured.<br />Results: The CP-HBV group exhibited lower bacterial shedding and an enhanced Th1-related immune response characterized by an upregulation of CD4+ T cells and CD4+ Interferon-γ+ T cells, accompanied by increased expression of Th1-related cytokines and reduced expression of regulatory T cells and immunosuppressive cytokines compared to the control group.<br />Clinical Relevance: CP-HBV is a promising strategy for controlling Salmonella infections in pigs and improving public health.
Details
- Language :
- English
- ISSN :
- 1943-5681
- Database :
- MEDLINE
- Journal :
- American journal of veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 38901460
- Full Text :
- https://doi.org/10.2460/ajvr.24.03.0086