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Ginsenoside Rb3 alleviates the formation of osteoclasts induced by periodontal ligament fibroblasts in the periodontitis microenvironment through the STAT3 pathway.
- Source :
-
Cell biology international [Cell Biol Int] 2024 Sep; Vol. 48 (9), pp. 1343-1353. Date of Electronic Publication: 2024 Jun 27. - Publication Year :
- 2024
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Abstract
- This study explores the potential role and mechanism of Ginsenoside Rb3 (Rb3) in modulating osteoclastogenesis induced by human periodontal ligament fibroblasts (hPLFs) within the periodontitis microenvironment. We investigated the anti-inflammatory effects of Rb3 on hPLFs stimulated with Porphyromonas gingivalis lipopolysaccharide (P.g-LPS) utilizing quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay techniques. Moreover, the functional role of Rb3 in hPLFs-induced osteoclast formation was assessed by treating human bone marrow-derived macrophages (hBMMs) with conditioned medium from hPLFs, followed by analyses through qPCR, western blot analysis, and staining for tartrate-resistant acid phosphatase (TRAP) and phalloidin. The impact of Rb3 on the activation of the STAT3 signaling pathway was determined via western blot analysis. Results indicated that Rb3 treatment significantly suppressed the upregulation of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, MCP-1, and IL-18) at both gene and protein levels in hPLFs induced by P.g-LPS. Furthermore, conditioned medium from Rb3 plus P.g-LPS treated hPLFs notably decreased the number of TRAP-positive cells, actin ring formations, and the expression of osteoclast marker genes (including CTSK, NFATC1, and ACP5). Rb3 also inhibited the P.g-LPS-induced activation of the STAT3 pathway, with the activation of STAT3 partially reversing the effects of Rb3 on inflammation and osteoclast differentiation. Collectively, Rb3 ameliorates inflammation in P.g-LPS-stimulated hPLFs and reduces hPLFs-induced osteoclastogenesis by inhibiting the STAT3 signaling pathway, suggesting its potential as a therapeutic agent for periodontitis.<br /> (© 2024 International Federation of Cell Biology.)
- Subjects :
- Humans
Cells, Cultured
Osteogenesis drug effects
Cell Differentiation drug effects
Lipopolysaccharides pharmacology
Porphyromonas gingivalis
Cellular Microenvironment drug effects
Cytokines metabolism
Macrophages metabolism
Macrophages drug effects
STAT3 Transcription Factor metabolism
Ginsenosides pharmacology
Fibroblasts metabolism
Fibroblasts drug effects
Osteoclasts drug effects
Osteoclasts metabolism
Periodontal Ligament cytology
Periodontal Ligament metabolism
Periodontal Ligament drug effects
Signal Transduction drug effects
Periodontitis metabolism
Periodontitis drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8355
- Volume :
- 48
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Cell biology international
- Publication Type :
- Academic Journal
- Accession number :
- 38934258
- Full Text :
- https://doi.org/10.1002/cbin.12201