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Bacterial Symbionts Contribute to Insecticide Susceptibility of Diaphorina citri via Changing the Expression Level of Host Detoxifying Genes.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Jul 10; Vol. 72 (27), pp. 15164-15175. Date of Electronic Publication: 2024 Jun 28. - Publication Year :
- 2024
-
Abstract
- Insecticide susceptibility is mainly determined by the insect host, but symbiotic bacteria are also an important affecting factor. In this study, we investigate the relationship between the structure of gut bacterial symbionts and insecticide susceptibility in Diaphorina citri , the important carrier of Candidatus Liberibacter asiaticus ( C Las), the causal agent of Huanglongbing (HLB). Our results indicated that antibiotic treatment significantly increased the susceptibility of D. citri to bifenthrin and thiamethoxam, and significantly decreased the relative abundance of Wolbachia and Profftella, enzyme activities of CarEs, and expression level of multiple CarE genes. The relative loads of Wolbachia and Profftella were positively correlated with DcitCCE13 , DcitCCE14 , DcitCCE15 , and DcitCCE16 . RNAi and prokaryotic expression revealed that DcitCCE15 is associated with bifenthrin metabolism. These results revealed that bacterial symbionts might regulate DcitCCE15 expression, which is involved in the susceptibility of D. citri to bifenthrin.
- Subjects :
- Animals
Insect Proteins genetics
Insect Proteins metabolism
Insecticide Resistance genetics
Wolbachia drug effects
Wolbachia genetics
Pyrethrins pharmacology
Bacteria genetics
Bacteria drug effects
Bacteria classification
Bacteria isolation & purification
Bacteria metabolism
Inactivation, Metabolic genetics
Symbiosis
Insecticides pharmacology
Hemiptera microbiology
Hemiptera genetics
Hemiptera drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 72
- Issue :
- 27
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 38938126
- Full Text :
- https://doi.org/10.1021/acs.jafc.4c03049