Back to Search
Start Over
Identification and serological responses to a novel Plasmodium vivax merozoite surface protein 1 ( Pv MSP-1) derived synthetic peptide: a putative biomarker for malaria exposure.
- Source :
-
PeerJ [PeerJ] 2024 Jun 25; Vol. 12, pp. e17632. Date of Electronic Publication: 2024 Jun 25 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Background: The integration of diagnostic methods holds promise for advancing the surveillance of malaria transmission in both endemic and non-endemic regions. Serological assays emerge as valuable tools to identify and delimit malaria transmission, serving as a complementary method to rapid diagnostic tests (RDT) and thick smear microscopy. Here, we evaluate the potential of antibodies directed against peptides encompassing the entire amino acid sequence of the Pv MSP-1 Sal-I strain as viable serological biomarkers for P. vivax exposure.<br />Methods: We screened peptides encompassing the complete amino acid sequence of the Plasmodium vivax Merozoite Surface Protein 1 ( Pv MSP-1) Sal-I strain as potential biomarkers for P. vivax exposure. Here, immunodominant peptides specifically recognized by antibodies from individuals infected with P. vivax were identified using the SPOT-synthesis technique followed by immunoblotting. Two 15-mer peptides were selected based on their higher and specific reactivity in immunoblotting assays. Subsequently, peptides p70 and p314 were synthesized in soluble form using SPPS (Solid Phase Peptide Synthesis) and tested by ELISA (IgG, and subclasses).<br />Results: This study unveils the presence of IgG antibodies against the peptide p314 in most P. vivax -infected individuals from the Brazilian Amazon region. In silico B-cell epitope prediction further supports the utilization of p314 as a potential biomarker for evaluating malaria transmission, strengthened by its amino acid sequence being part of a conserved block of Pv MSP-1. Indeed, compared to patients infected with P. falciparum and uninfected individuals never exposed to malaria, P. vivax -infected patients have a notably higher recognition of p314 by IgG1 and IgG3.<br />Competing Interests: Erika M. Braga is an Academic Editor for PeerJ.<br /> (©2024 Marzano-Miranda et al.)
- Subjects :
- Humans
Immunoglobulin G immunology
Immunoglobulin G blood
Adult
Female
Male
Middle Aged
Peptides immunology
Enzyme-Linked Immunosorbent Assay methods
Young Adult
Adolescent
Amino Acid Sequence
Malaria, Vivax immunology
Malaria, Vivax blood
Malaria, Vivax parasitology
Malaria, Vivax transmission
Malaria, Vivax diagnosis
Merozoite Surface Protein 1 immunology
Plasmodium vivax immunology
Biomarkers blood
Antibodies, Protozoan immunology
Antibodies, Protozoan blood
Subjects
Details
- Language :
- English
- ISSN :
- 2167-8359
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- PeerJ
- Publication Type :
- Academic Journal
- Accession number :
- 38948214
- Full Text :
- https://doi.org/10.7717/peerj.17632