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Retrotransposon-mediated disruption of a chitin synthase gene confers insect resistance to Bacillus thuringiensis Vip3Aa toxin.
- Source :
-
PLoS biology [PLoS Biol] 2024 Jul 02; Vol. 22 (7), pp. e3002704. Date of Electronic Publication: 2024 Jul 02 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- The vegetative insecticidal protein Vip3Aa from Bacillus thuringiensis (Bt) has been produced by transgenic crops to counter pest resistance to the widely used crystalline (Cry) insecticidal proteins from Bt. To proactively manage pest resistance, there is an urgent need to better understand the genetic basis of resistance to Vip3Aa, which has been largely unknown. We discovered that retrotransposon-mediated alternative splicing of a midgut-specific chitin synthase gene was associated with 5,560-fold resistance to Vip3Aa in a laboratory-selected strain of the fall armyworm, a globally important crop pest. The same mutation in this gene was also detected in a field population. Knockout of this gene via CRISPR/Cas9 caused high levels of resistance to Vip3Aa in fall armyworm and 2 other lepidopteran pests. The insights provided by these results could help to advance monitoring and management of pest resistance to Vip3Aa.<br />Competing Interests: The authors have declared that no competing interests exist<br /> (Copyright: © 2024 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Subjects :
- Animals
CRISPR-Cas Systems
Alternative Splicing genetics
Alternative Splicing drug effects
Spodoptera drug effects
Plants, Genetically Modified
Moths drug effects
Moths genetics
Bacterial Proteins genetics
Bacterial Proteins metabolism
Chitin Synthase genetics
Chitin Synthase metabolism
Retroelements genetics
Bacillus thuringiensis genetics
Insecticide Resistance genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1545-7885
- Volume :
- 22
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- PLoS biology
- Publication Type :
- Academic Journal
- Accession number :
- 38954724
- Full Text :
- https://doi.org/10.1371/journal.pbio.3002704