Back to Search
Start Over
Antibacterial sequential growth factor delivery from alginate/gelatin methacryloyl microspheres for bone regeneration.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2024 Aug; Vol. 275 (Pt 1), pp. 133557. Date of Electronic Publication: 2024 Jun 30. - Publication Year :
- 2024
-
Abstract
- Autologous or allogeneic bone tissue grafts remain the mainstay of treatment for clinical bone defects. However, the risk of infection and donor scarcity in bone grafting pose challenges to the process. Therefore, the development of excellent biomaterial grafts is of great clinical importance for the repair of bone defects. In this study, we used gas-assisted microfluidics to construct double-cross-linked hydrogel microspheres with good biological function based on the ionic cross-linking of Cu <superscript>2+</superscript> with alginate and photo-cross-linking of gelatin methacryloylamide (GelMA) by loading vascular endothelial growth factor (VEGF) and His-tagged bone morphogenetic protein-2 (BMP2) (AGMP@VEGF&BMP2). The Cu <superscript>2+</superscript> component in the microspheres showed good antibacterial and drug-release behavior, whereas VEGF and BMP2 effectively promoted angiogenesis and bone tissue repair. In in vitro and in vivo experiments, the dual cross-linked hydrogel microspheres showed good biological function and biocompatibility. These results demonstrate that AGMP@VEGF&BMP2 microspheres could be used as a bone defect graft substitute to promote effective healing of bone defects and may be applied to other tissue engineering studies.<br />Competing Interests: Declaration of competing interest All authors disclosed no relevant relationships. This manuscript has not been published or presented elsewhere in part or in entirety and is not under consideration by another journal. We have read and understood your journal's policies, and we believe that neither the manuscript nor the study violates any of these. There are no conflicts of interest to declare.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Vascular Endothelial Growth Factor A pharmacology
Vascular Endothelial Growth Factor A chemistry
Vascular Endothelial Growth Factor A metabolism
Methacrylates chemistry
Methacrylates pharmacology
Mice
Hydrogels chemistry
Hydrogels pharmacology
Humans
Drug Liberation
Gelatin chemistry
Gelatin pharmacology
Microspheres
Alginates chemistry
Alginates pharmacology
Bone Regeneration drug effects
Anti-Bacterial Agents pharmacology
Anti-Bacterial Agents chemistry
Bone Morphogenetic Protein 2 pharmacology
Bone Morphogenetic Protein 2 chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 275
- Issue :
- Pt 1
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 38955293
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2024.133557