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Molecular based identification and phylogenetic relationship of the leech Hirudinaria manillensis from India by using mitochondrial cytochrome c oxidase subunit I gene.
- Source :
-
Molecular biology reports [Mol Biol Rep] 2024 Jul 06; Vol. 51 (1), pp. 787. Date of Electronic Publication: 2024 Jul 06. - Publication Year :
- 2024
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Abstract
- Background: A molecular approach for the identification of unknown species by the using mitochondrial cox1 gene is an effective and reliable as compared with morphological-based identification. Hirudinaria manillensis referred to as Asian Buffalo Leech, is found in South Asia and traditionally used as medicine owing to its medicinal properties.<br />Methods and Results: The study aimed to isolate and identify the leech species using cox1 gene sequencing and their phylogenetic relationships. The nucleotide sequences of cytochrome c oxidase subunit I (cox1) mitochondrial genes were analyzed for species identification and the phylogenetic relationship of crucial therapeutic leech Hirudinaria manillensis. The isolated DNA from the leech sample was amplified with cox1 gene-specific primers. BLAST results with the H. manillensis sequence showed 89.24% homology with H. manillensis and phylogenetic tree analysis revealed the genetic relationship with other GenBank submitted sequences.<br />Conclusion: The present study concluded that the cox1 gene could be an effective way to identify the leech H. manillensis and provided sufficient phylogenetic information to distinguish H. manillensis indicating a significant mtDNA-based approach to species identification.<br /> (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Details
- Language :
- English
- ISSN :
- 1573-4978
- Volume :
- 51
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular biology reports
- Publication Type :
- Academic Journal
- Accession number :
- 38970720
- Full Text :
- https://doi.org/10.1007/s11033-024-09720-1