Back to Search
Start Over
PLA plastic particles disrupt bile acid metabolism leading to hepatic inflammatory injury in male mice.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Nov 05; Vol. 732, pp. 150410. Date of Electronic Publication: 2024 Jul 17. - Publication Year :
- 2024
-
Abstract
- Microplastics, such as polylactic acid (PLA), are ubiquitous environmental pollutants with unclear implications for health impact. This study aims to elucidate the mechanisms of PLA-induced inflammatory liver injury, focusing on disturbance of bile acid metabolism. The in vitro PLA exposure experiment was conducted using HepG2 cells to assess cell viability, cytokine secretion, and effects on bile acid metabolism. In vivo, male C57BL/6 J mice were exposed to PLA for ten days continuously, liver function and histopathological assessment were evaluated after the mice sacrificed. Molecular analyses including quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blotting, were applied to evaluate the expression of bile acid metabolizing enzymes and transporters. PLA exposure resulted in decreased cell viability in HepG2 cells, increased inflammation and altered bile acid metabolism. In mice, PLA exposure resulted in decreased body weight and food intake, impaired liver function, increased hepatic inflammation, altered bile acid profiles, and dysregulated expression of bile acid metabolic pathways. PLA exposure disrupts bile acid metabolism through inhibition of the CYP7A1 enzyme and activation of the FGF-JNK/ERK signaling pathway, contributing to liver injury. These findings highlight the potential hepatotoxic effects of environmentally friendly plastics PLA and underscore the need for further research on their biological impact.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence that work reported in this paper.<br /> (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Male
Humans
Hep G2 Cells
Mice
Liver metabolism
Liver pathology
Liver drug effects
Microplastics toxicity
Chemical and Drug Induced Liver Injury metabolism
Chemical and Drug Induced Liver Injury pathology
Chemical and Drug Induced Liver Injury etiology
Cholesterol 7-alpha-Hydroxylase metabolism
Cholesterol 7-alpha-Hydroxylase genetics
Cell Survival drug effects
Inflammation metabolism
Inflammation pathology
Bile Acids and Salts metabolism
Mice, Inbred C57BL
Polyesters
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 732
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 39032413
- Full Text :
- https://doi.org/10.1016/j.bbrc.2024.150410