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Exploring the nuclear proteins, viral capsid protein, and early antigen protein using immunoinformatic and molecular modeling approaches to design a vaccine candidate against Epstein Barr virus.
- Source :
-
Scientific reports [Sci Rep] 2024 Jul 22; Vol. 14 (1), pp. 16798. Date of Electronic Publication: 2024 Jul 22. - Publication Year :
- 2024
-
Abstract
- The available Epstein Barr virus vaccine has tirelessly harnessed the gp350 glycoprotein as its target epitope, but the result has not been preventive. Right here, we designed a global multi-epitope vaccine for EBV; with special attention to making sure all strains and preventive antigens are covered. Using a robust computational vaccine design approach, our proposed vaccine is armed with 6-16 mers linear B-cell epitopes, 4-9 mer CTL epitopes, and 8-15 mer HTL epitopes which are verified to induce interleukin 4, 10 & IFN-gamma. We employed deep computational mining coupled with expert intelligence in designing the vaccine, using human Beta defensin-3-which has been reported to induce the same TLRs as EBV-as the adjuvant. The tendency of the vaccine to cause autoimmune disorder is quenched by the assurance that the construct contains no EBNA-1 homolog. The protein vaccine construct exhibited excellent physicochemical attributes such as Aliphatic index 59.55 and GRAVY - 0.710; and a ProsaWeb Z score of - 3.04. Further computational analysis revealed the vaccine docked favorably with EBV indicted TLR 1, 2, 4 & 9 with satisfactory interaction patterns. With global coverage of 85.75% and the stable molecular dynamics result obtained for the best two interactions, we are optimistic that our nontoxic, non-allergenic multi-epitope vaccine will help to ameliorate the EBV-associated diseases-which include various malignancies, tumors, and cancers-preventively.<br /> (© 2024. The Author(s).)
- Subjects :
- Humans
Epstein-Barr Virus Infections immunology
Epstein-Barr Virus Infections prevention & control
Epitopes, B-Lymphocyte immunology
Computational Biology methods
Epitopes, T-Lymphocyte immunology
Viral Vaccines immunology
Antigens, Viral immunology
Antigens, Viral chemistry
Models, Molecular
Molecular Docking Simulation
Herpesvirus 4, Human immunology
Capsid Proteins immunology
Capsid Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 39039173
- Full Text :
- https://doi.org/10.1038/s41598-024-66828-x