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Structural characterisation and anti-colon cancer activity of an arabinogalactan RSA-1 from Raphani semen.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2024 Oct 15; Vol. 342, pp. 122417. Date of Electronic Publication: 2024 Jun 18. - Publication Year :
- 2024
-
Abstract
- RSA-1 is a polysaccharide obtained from Raphani semen with a relatively clear structure and anti-colon cancer activity. In this study, high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy were applied to characterise the complex chain structure of RSA-1. Subsequently, the inhibitory effect on colon cancer growth through apoptosis induction in colon cancer cells was explored. The findings indicate that the main chain of RSA-1 consists of →3)-β-D-Galp-(1 → and 3,6)-β-D-Galp-(1 → substituted at C-6 with branched α-L-Araf side chains. RSA-1 disrupts the Bax/Bcl-2 ratio and thus inhibits the viability of colon cancer cells in vitro. Furthermore, it inhibits colon cancer migration by attenuating epithelial-mesenchymal transition. Notably, RSA-1 exhibited negligible impact on the growth of human intestinal epithelial cells within a relevant concentration range. This study establishes a theoretical foundation and provides technical support for the prospective development and application of RSA-1 as a dual-purpose anti-colon cancer drug and functional food.<br />Competing Interests: Declaration of competing interest There was no conflict of interest in the submission of this original research article, and all authors have given their approval for its publication.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Humans
Apoptosis drug effects
Cell Proliferation drug effects
Antineoplastic Agents, Phytogenic pharmacology
Antineoplastic Agents, Phytogenic chemistry
Antineoplastic Agents, Phytogenic isolation & purification
Cell Line, Tumor
Cell Movement drug effects
Cell Survival drug effects
Epithelial-Mesenchymal Transition drug effects
Galactans chemistry
Galactans pharmacology
Galactans isolation & purification
Colonic Neoplasms drug therapy
Colonic Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 342
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 39048243
- Full Text :
- https://doi.org/10.1016/j.carbpol.2024.122417