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Hexane insoluble fraction from purple rice extract improves steatohepatitis and fibrosis via inhibition of NF-κB and JNK signaling.
- Source :
-
Food & function [Food Funct] 2024 Aug 12; Vol. 15 (16), pp. 8562-8571. Date of Electronic Publication: 2024 Aug 12. - Publication Year :
- 2024
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Abstract
- Nonalcoholic fatty liver disease (NAFLD) is fatty liver mainly related to metabolic syndrome. NAFLD with inflammation and hepatocellular damage is defined as nonalcoholic steatohepatitis (NASH), which can progress to cirrhosis and hepatocellular carcinoma. We have previously reported that a hexane insoluble fraction from an anthocyanin-rich purple rice ethanolic extract (PRE-HIF) can suppress prostate carcinogenesis. However, the extract's effect on NASH has not yet been established. In the present study, we investigated the chemopreventive effect of a PRE-HIF on NASH and liver fibrosis using a connexin 32 (Cx32) dominant negative transgenic (Cx32ΔTg) rat NASH model. Seven-week-old male Cx32ΔTg rats were fed a control diet, a high-fat diet (HFD), or an HFD with 1% PRE-HIF and intraperitoneal administration of dimethylnitrosamine for 17 weeks. Histological findings of NASH such as fat deposition, lobular inflammation, hepatocyte ballooning injury, and bridging fibrosis were observed in the HFD group but not in the control group, and all histological parameters were significantly improved by PRE-HIF treatment. Corresponding to the histological changes, increased expression of inflammatory cytokine mRNAs (TNF-α, IL-6, IL-18, IFN-γ, IL-1β, TGF-β1, TIMP1, TIMP2, COL1A1), along with and activation of nuclear factor-κB (NF-κB) and c-Jun N-terminal kinase (JNK) signaling were observed in the HFD group, which was significantly decreased by PRE-HIF. The number and area of hepatic precancerous glutathione S -transferase placental form-positive foci tended to be decreased by PRE-HIF. These results indicate that intake of purple rice as a dietary supplement may reduce steatohepatitis, liver injury, and fibrosis in NASH by inactivation of NF-κB or JNK.
- Subjects :
- Animals
Male
Rats
Humans
Liver drug effects
Liver metabolism
Liver pathology
MAP Kinase Signaling System drug effects
Diet, High-Fat adverse effects
Disease Models, Animal
Signal Transduction drug effects
Cytokines metabolism
Cytokines genetics
NF-kappa B metabolism
NF-kappa B genetics
Plant Extracts pharmacology
Non-alcoholic Fatty Liver Disease drug therapy
Non-alcoholic Fatty Liver Disease metabolism
Liver Cirrhosis drug therapy
Liver Cirrhosis metabolism
Oryza chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2042-650X
- Volume :
- 15
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Food & function
- Publication Type :
- Academic Journal
- Accession number :
- 39072556
- Full Text :
- https://doi.org/10.1039/d4fo00292j