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Pro-apoptotic gene BAX is a pan-cancer predictive biomarker for prognosis and immunotherapy efficacy.
- Source :
-
Aging [Aging (Albany NY)] 2024 Jul 05; Vol. 16 (14), pp. 11289-11317. Date of Electronic Publication: 2024 Jul 05. - Publication Year :
- 2024
-
Abstract
- Background: Apoptosis Regulator BCL2 Associated X (BAX) is a pro-apoptotic gene. Apoptosis is one of the important components of immune response and immune regulation. However, there is no systematic pan-cancer analysis of BAX.<br />Methods: Original data of this study were downloaded from TCGA databases and GTEX databases. We conducted the gene expression analysis and survival analysis of BAX in 33 types of cancer via Gene Expression Profiling Interactive Analysis (GEPIA) database. Real-time PCR and immunohistochemistry (IHC) were further performed to examine the BAX expression in cancer cells and tissues. Moreover, the relationship between BAX and immune infiltration and gene alteration was studied by the Tumor Immune Estimation Resource (TIMER) and cBioPortal tools. Protein-protein interaction analysis was performed in the STRING database. Finally, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were utilized to evaluate the enrichment analysis.<br />Results: BAX was highly expressed in most cancers and was associated with poor prognosis in nine cancer types. In addition, BAX showed significant clinical relevance, and the mRNA expression of BAX was also strongly associated with drug sensitivity of many drugs. Furthermore, BAX may participate in proliferation and metastasis of many cancers and was associated with methylation. Importantly, BAX expression was positively correlated with most immune infiltrating cells.<br />Conclusion: Our findings suggested that BAX can function as an oncogene and may be used as a potential predictive biomarker for prognosis and immunotherapy efficacy of human cancer, which could provide a new approach for cancer therapy.
- Subjects :
- Humans
Prognosis
Apoptosis genetics
Gene Expression Profiling
Databases, Genetic
Protein Interaction Maps
bcl-2-Associated X Protein genetics
bcl-2-Associated X Protein metabolism
Neoplasms genetics
Neoplasms immunology
Neoplasms therapy
Biomarkers, Tumor genetics
Biomarkers, Tumor metabolism
Immunotherapy methods
Gene Expression Regulation, Neoplastic
Subjects
Details
- Language :
- English
- ISSN :
- 1945-4589
- Volume :
- 16
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Aging
- Publication Type :
- Academic Journal
- Accession number :
- 39074253
- Full Text :
- https://doi.org/10.18632/aging.206003