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Co-transcriptional production of programmable RNA condensates and synthetic organelles.
- Source :
-
Nature nanotechnology [Nat Nanotechnol] 2024 Jul 30. Date of Electronic Publication: 2024 Jul 30. - Publication Year :
- 2024
- Publisher :
- Ahead of Print
-
Abstract
- Condensation of RNA and proteins is central to cellular functions, and the ability to program it would be valuable in synthetic biology and synthetic cell science. Here we introduce a modular platform for engineering synthetic RNA condensates from tailor-made, branched RNA nanostructures that fold and assemble co-transcriptionally. Up to three orthogonal condensates can form simultaneously and selectively accumulate fluorophores through embedded fluorescent light-up aptamers. The RNA condensates can be expressed within synthetic cells to produce membrane-less organelles with a controlled number and relative size, and showing the ability to capture proteins using selective protein-binding aptamers. The affinity between otherwise orthogonal nanostructures can be modulated by introducing dedicated linker constructs, enabling the production of bi-phasic RNA condensates with a prescribed degree of interphase mixing and diverse morphologies. The in situ expression of programmable RNA condensates could underpin the spatial organization of functionalities in both biological and synthetic cells.<br /> (© 2024. The Author(s).)
Details
- Language :
- English
- ISSN :
- 1748-3395
- Database :
- MEDLINE
- Journal :
- Nature nanotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 39080489
- Full Text :
- https://doi.org/10.1038/s41565-024-01726-x