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Genome-wide identification, abiotic stress, and expression analysis of PYL family in Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) during grain development.
- Source :
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BMC plant biology [BMC Plant Biol] 2024 Jul 30; Vol. 24 (1), pp. 725. Date of Electronic Publication: 2024 Jul 30. - Publication Year :
- 2024
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Abstract
- Background: Abscisic acid (ABA) is a plant hormone that plays an important role in plant resistance to drought, salinity, cold, and pathogens. It is also important for regulating plant growth and development. Pyrabactin resistance/pyr1-like/regulatory components of the ABA receptor (PYL/RCAR) are ABA receptor proteins in plants and the core of ABA signal transduction pathways in plant regulatory factors. At present, there are no reports on the PYL family of Tartary buckwheat.<br />Results: In this study, 19 paralogous form PYL genes in buckwheat were identified at the whole-genome level and named FtPYL1-FtPYL19 according to their positions on chromosomes. We further analyzed the gene structure, conserved motifs, cis-acting elements, gene duplication, phylogenetic relationships, and expression patterns under different stress treatments and during grain development of the 19 paralogous form PYL genes in Tartary buckwheat. The FtPYL gene exhibits a single exonic gene structure for about 68.4% of the duplicated forms from the total paralogous forms. The remaining subfamilies, such as I and II, contain three exons and two exons (e.g., FtPYL19), respectively. Nineteen FtPYL genes were evenly distributed across the eight chromosomes, with at least one FtPYL gene on each chromosome. In the FtPYL gene family, there was one tandem repeat event and five gene duplication events. We investigated the gene expression levels of FtPYL gene under four abiotic stresses and different stages of grain development. Under drought stress (PEG6000), the relative expression levels of FtPYL14 and FtPYL15 increased by fourfold. Under high temperature stress (38℃), the relative expression level of FtPYL16 dropped to 0.12, and that of FtPYL17 fell to 0.22. At different stages of grain development, the gene expression level of FtPY15 is extremely high at 19 D. The relative expression level of FtPYL7 in roots and stems reaches up to approximately 450, and the relative expression level of FtPYL10 in 13 D also reaches up to 248. In this study, the PYL gene family of Tartary buckwheat was identified and analyzed based on the whole genome, and 19 paralogous form FtPYL genes of Tartary buckwheat were bioinformatically analyzed. The expression patterns of 19 paralogous form FtPYL genes in Tartary buckwheat cultivars under different stress treatments and during grain development were analyzed. It was found that the FtPYL gene played an important role in grain development.<br /> (© 2024. The Author(s).)
- Subjects :
- Multigene Family
Genome, Plant
Edible Grain genetics
Edible Grain growth & development
Gene Duplication
Genes, Plant
Abscisic Acid metabolism
Fagopyrum genetics
Fagopyrum growth & development
Plant Proteins genetics
Plant Proteins metabolism
Stress, Physiological genetics
Phylogeny
Gene Expression Regulation, Plant
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2229
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC plant biology
- Publication Type :
- Academic Journal
- Accession number :
- 39080537
- Full Text :
- https://doi.org/10.1186/s12870-024-05447-0