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Efficient fabrication of 3D bioprinted functional sensory neurons using an inducible Neurogenin-2 human pluripotent stem cell line.
- Source :
-
Biofabrication [Biofabrication] 2024 Aug 14; Vol. 16 (4). Date of Electronic Publication: 2024 Aug 14. - Publication Year :
- 2024
-
Abstract
- Three-dimensional (3D) tissue models have gained recognition for their improved ability to mimic the native cell microenvironment compared to traditional two-dimensional models. This progress has been driven by advances in tissue-engineering technologies such as 3D bioprinting, a promising method for fabricating biomimetic living tissues. While bioprinting has succeeded in generating various tissues to date, creating neural tissue models remains challenging. In this context, we present an accelerated approach to fabricate 3D sensory neuron (SN) structures using a transgenic human pluripotent stem cell (hPSC)-line that contains an inducible Neurogenin-2 (NGN2) expression cassette. The NGN2 hPSC line was first differentiated to neural crest cell (NCC) progenitors, then incorporated into a cytocompatible gelatin methacryloyl-based bioink for 3D bioprinting. Upregulated NGN2 expression in the bioprinted NCCs resulted in induced SN (iSN) populations that exhibited specific cell markers, with 3D analysis revealing widespread neurite outgrowth through the scaffold volume. Calcium imaging demonstrated functional activity of iSNs, including membrane excitability properties and voltage-gated sodium channel (Na <subscript>V</subscript> ) activity. This efficient approach to generate 3D bioprinted iSN structures streamlines the development of neural tissue models, useful for the study of neurodevelopment and disease states and offering translational potential.<br /> (Creative Commons Attribution license.)
- Subjects :
- Humans
Cell Line
Pluripotent Stem Cells cytology
Pluripotent Stem Cells metabolism
Cell Differentiation
Tissue Engineering methods
Gelatin chemistry
Neural Crest cytology
Neural Crest metabolism
Printing, Three-Dimensional
Bioprinting methods
Basic Helix-Loop-Helix Transcription Factors metabolism
Basic Helix-Loop-Helix Transcription Factors genetics
Sensory Receptor Cells metabolism
Sensory Receptor Cells cytology
Nerve Tissue Proteins metabolism
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1758-5090
- Volume :
- 16
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biofabrication
- Publication Type :
- Academic Journal
- Accession number :
- 39084624
- Full Text :
- https://doi.org/10.1088/1758-5090/ad69c4