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Evaluation of Neuroprotective Effect of Gut Microbe in Parkinson's Disease: An In Silico and In Vivo Approach.
- Source :
-
Neuromolecular medicine [Neuromolecular Med] 2024 Aug 01; Vol. 26 (1), pp. 32. Date of Electronic Publication: 2024 Aug 01. - Publication Year :
- 2024
-
Abstract
- Parkinson's disease is a progressive neurodegenerative disorder marked by the death of dopaminergic neurons in the substantia nigra region of the brain. Aggregation of alpha-synuclein (α-synuclein) is a contributing factor to Parkinson's disease pathogenesis. The objective of this study is to investigate the neuroprotective effects of gut microbes on α-synuclein aggregation using both in silico and in vivo approaches. We focussed on the interaction between α-synuclein and metabolites released by gut bacteria that protect from PD. We employed three probiotic microbe strains against α-synuclein protein: Lactobacillus casei, Escherichia coli, and Bacillus subtilis, with their chosen PDB IDs being Dihydrofolate reductase (3DFR), methionine synthetase (6BM5), and tryptophanyl-tRNA synthetase (3PRH), respectively. Using HEX Dock 6.0 software, we examined the interactions between these proteins. Among the various metabolites, methionine synthetase produced by E. coli showed potential interactions with α-synuclein. To further evaluate the neuroprotective benefits of E. coli, an in vivo investigation was performed using a rotenone-induced Parkinsonian mouse model. The motor function of the animals was assessed through behavioural tests, and oxidative stress and neurotransmitter levels were also examined. The results demonstrated that, compared to the rotenone-induced PD mouse model, the rate of neurodegeneration was considerably reduced in mice treated with E. coli. Additionally, histopathological studies provided evidence of the neuroprotective effects of E. coli. In conclusion, this study lays the groundwork for future research, suggesting that gut bacteria may serve as potential therapeutic agents in the development of medications to treat Parkinson's disease. fig. 1.<br /> (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Animals
Mice
Lacticaseibacillus casei physiology
Methionine-tRNA Ligase
Tryptophan-tRNA Ligase physiology
Male
Tetrahydrofolate Dehydrogenase metabolism
Computer Simulation
Parkinsonian Disorders microbiology
Humans
Neuroprotective Agents therapeutic use
Mice, Inbred C57BL
Disease Models, Animal
Parkinson Disease, Secondary chemically induced
Dopaminergic Neurons drug effects
Parkinson Disease microbiology
Gastrointestinal Microbiome physiology
Probiotics therapeutic use
Probiotics pharmacology
alpha-Synuclein metabolism
Escherichia coli
Bacillus subtilis
Molecular Docking Simulation
Oxidative Stress drug effects
Rotenone toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1559-1174
- Volume :
- 26
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuromolecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 39090268
- Full Text :
- https://doi.org/10.1007/s12017-024-08799-w