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Lipopolysaccharide-induced bacterial infection model: microRNA-370-3p participates in the anti-infection response by targeting the macrophage TLR4-NLRP3 caspase-1 cellular pyroptosis pathway.
- Source :
-
International journal of immunopathology and pharmacology [Int J Immunopathol Pharmacol] 2024 Jan-Dec; Vol. 38, pp. 3946320241272550. - Publication Year :
- 2024
-
Abstract
- Objective: To explore the effect of miR-370-3p on LPS triggering, in particular its involvement in disease progression by targeting the TLR4-NLRP3-caspase-1 cellular pyroptosis pathway in macrophages.<br />Methods: Human macrophage RAW264.7 was divided into 6 groups: control, LPS, LPS + inhibitor-NC, LPS + miR-370-3p inhibitor, LPS + mimics-NC and LPS + miR-370-3p mimics. RT-qPCR was used to detect the expression level of miR-370-3p and analyzed comparatively. CCK-8 and flow cytometry assays were used to detect cell viability and apoptosis. ELISA assay was used to detect the levels of IL-1β and TNF-α in the supernatant of the cells. The WB assay was used to detect TLR4, NLRP3, Caspase-1 and GSDMD levels.<br />Results: After LPS induction, macrophage miR-370-3p levels decreased, cell viability decreased, and apoptosis increased. At the same time, the levels of TLR4, NLRP3, Caspase-1 and GSDMD increased in the cells, and the levels of IL-1β and TNF-α increased in the cell supernatant. Compared with the LPS group, the significantly higher expression level of miR-370-3p in the cells of the LPS + miR-370-3p mimics group was accompanied by significantly higher cell viability, significantly lower apoptosis rate, significantly lower levels of TLR4, NLRP3, Caspase-1, and GSDMD in the cells, and significantly lower levels of IL-1β and TNF-α in the cell supernatant.<br />Conclusion: MiR-370-3p may be involved in anti-infective immune responses by targeting and inhibiting the macrophage TLR4-NLRP3-caspase-1 cellular pyroptosis pathway.<br />Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Subjects :
- Humans
Mice
RAW 264.7 Cells
Animals
Signal Transduction
Interleukin-1beta metabolism
Cell Survival drug effects
Bacterial Infections immunology
MicroRNAs genetics
MicroRNAs metabolism
NLR Family, Pyrin Domain-Containing 3 Protein metabolism
NLR Family, Pyrin Domain-Containing 3 Protein genetics
Toll-Like Receptor 4 metabolism
Toll-Like Receptor 4 genetics
Pyroptosis
Lipopolysaccharides pharmacology
Macrophages metabolism
Macrophages immunology
Macrophages drug effects
Caspase 1 metabolism
Caspase 1 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2058-7384
- Volume :
- 38
- Database :
- MEDLINE
- Journal :
- International journal of immunopathology and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 39101927
- Full Text :
- https://doi.org/10.1177/03946320241272550